Hasan H A
Botany Department, Faculty of Science, Assiut University, Egypt.
J Nat Toxins. 2001 May;10(2):127-36.
The research is conducted on the effect of pencycuron and pencycuron-c on cotton seed mycoflora, aflatoxin production and viability. At 8% seed moisture content (mc), pencycuron and pencycuron-c promoted Aspergillus niger, A. flavus, and Penicillium corylophilum growth count at 1 g/kg, but exerted inhibitory effect at 3 and 5 g/kg. At 15% mc, pencycuron enhanced seed-borne fungi at all three doses after most treatment periods (1, 2, 3, 4, and 5 months), whereas pencycuron-c induced inhibition effect. The A. niger utilized pencycuron as nitrogen source more than pencycuron-c. Seeds with 15% mc lost their viability faster than that at 8%, and this was more evident as storage time increased. Such loss occurred faster when seed was treated with pencycuron, whereas pencycuron-c exerted significant activation in the viability compared to the control. The fungal species have high biodegradation activity and produce aflatoxin in different parts of cotton boll (fiber, valves, and seeds). Pencycuron and pencycuron-c inhibited aflatoxin B1 and B2 production in seeds, but did not affect aflatoxin G1 and G2.
本研究针对拌种灵及拌种灵 - c 对棉花种子真菌区系、黄曲霉毒素产生及活力的影响展开。在种子含水量为 8%时，拌种灵及拌种灵 - c 在 1 g/kg 剂量下促进了黑曲霉、黄曲霉和嗜松青霉的生长，但在 3 g/kg 和 5 g/kg 剂量下产生抑制作用。在含水量为 15%时，在大多数处理时期（1、2、3、4 和 5 个月），拌种灵在所有三个剂量下均增强了种子携带真菌的数量，而拌种灵 - c 则产生抑制作用。黑曲霉利用拌种灵作为氮源的能力强于拌种灵 - c。含水量为 15%的种子比含水量为 8%的种子更快丧失活力，且随着储存时间延长这种情况更为明显。用拌种灵处理种子时，这种活力丧失更快，而与对照相比，拌种灵 - c 对种子活力有显著激活作用。这些真菌物种具有高生物降解活性，并在棉铃的不同部位（纤维、棉籽壳和种子）产生黄曲霉毒素。拌种灵和拌种灵 - c 抑制种子中黄曲霉毒素 B1 和 B2 的产生，但不影响黄曲霉毒素 G1 和 G2。
