使用本地分离的腐生性钩端螺旋体菌株进行人类钩端螺旋体病的血清学检测。

Use of locally isolated saprophytic Leptospira strain for serological testing of human leptospirosis.

作者信息

Sharma R, Tuteja U, Batra H V

机构信息

Department of Microbiology, Defence Reaserch and Establishment, Gwalior, M.P.

出版信息

J Commun Dis. 2000 Sep;32(3):185-9.

PMID:11407004

Abstract

A saprophytic Leptospira isolate recovered from tap water was utilized for serological testing. One hundred-twenty Serum samples comprising 55 cases from PUO/febrile jaundice and 65 samples from apparently healthy individuals were tested by MAT and HA using this environmental saprophytic strain and the results compared with that of Leptospira biflexa semaranga patoc, the standard saprophytic strain commonly employed for sero-diagnosis of leptospirosis. The MAT data showed 96.4 per cent correlation between the two strains. Similarly, the HA results were matching to the extent of 94.5 per cent. Results, therefore, suggest that local saprophytic Leptospira strain may serve as a substitute to serovar patoc for serodiagnosis of leptospirosis.

摘要

从自来水中分离出的一株腐生性钩端螺旋体用于血清学检测。使用这种环境腐生菌株，通过显微镜凝集试验（MAT）和血凝试验（HA）对120份血清样本进行检测，其中包括55例不明原因发热/黄疸病例的样本和65例看似健康个体的样本，并将结果与双曲钩端螺旋体塞马朗帕托克（Leptospira biflexa semaranga patoc）（通常用于钩端螺旋体病血清诊断的标准腐生菌株）的结果进行比较。MAT数据显示这两种菌株之间的相关性为96.4%。同样，HA结果的匹配程度为94.5%。因此，结果表明本地腐生钩端螺旋体菌株可替代帕托克血清型用于钩端螺旋体病的血清诊断。