合成肽来源于 Hap1/LipL32，用于人类钩端螺旋体病的早期血清学诊断。
Synthetic peptide issued from Hap1/LipL32 for new early serodiagnosis of human leptospirosis.
机构信息
Unité de Bactériologie Médicale et Moléculaire des Leptospires, Ecole Nationale Vétérinaire de Nantes, BP 40 706, 44 307 Nantes Cedex 03, France.
出版信息
Comp Immunol Microbiol Infect Dis. 2010 Sep;33(5):375-87. doi: 10.1016/j.cimid.2009.01.001. Epub 2009 Mar 23.
Leptospirosis is a worldwide zoonosis. Today, serological diagnosis is generally assessed by MAT. We performed ELISA with a synthetic peptide derived from Hap1/lipL32 which is a protein expressed only by pathogenic Leptospira. Repeatability and thresholds were defined with 85 controls sera and 119 hospitalized leptospirosis. The PP-ELISA repeatability and IgM/IgG cut-off values were based on control sera. For these cut-off values, we observed the IgM-PP-ELISA specificity of 89%, whereas it was 100% for the IgG. Then, we compared PP-ELISA and standard MAT results for leptospirosis patients. The concordance rate for IgM-PP-ELISA and MAT was low (43%), whereas it was 85% for IgG-PP-ELISA and MAT. During the first 5 days after hospitalization, PP-ELISA gave positive results in 13 out of 16 patients (81%) whereas 8 out of 14 patients (57%) were positive to MAT. ELISA using Hap1/lipL 32-derived synthetic peptide PP is an earlier serological diagnosis of human leptospirosis than MAT.
钩端螺旋体病是一种世界性的人畜共患病。如今，血清学诊断通常通过 MAT 进行评估。我们使用源自仅由致病性钩端螺旋体表达的蛋白 Hap1/lipL32 的合成肽进行 ELISA。用 85 份对照血清和 119 份住院钩端螺旋体病血清来定义重复性和阈值。PP-ELISA 的重复性和 IgM/IgG 截断值基于对照血清确定。对于这些截断值，我们观察到 IgM-PP-ELISA 的特异性为 89%，而 IgG 的特异性为 100%。然后，我们比较了 PP-ELISA 和标准 MAT 对钩端螺旋体病患者的结果。IgM-PP-ELISA 和 MAT 的一致性率较低（43%），而 IgG-PP-ELISA 和 MAT 的一致性率为 85%。在住院后的前 5 天，PP-ELISA 在 16 名患者中的 13 名（81%）中产生阳性结果，而 MAT 在 14 名患者中的 8 名（57%）中产生阳性结果。使用源自 Hap1/lipL 32 的合成肽 PP 的 ELISA 是比 MAT 更早的人类钩端螺旋体病血清学诊断方法。
Zotero 插件