Unité de Bactériologie Médicale et Moléculaire des Leptospires, Ecole Nationale Vétérinaire de Nantes, BP 40 706, 44 307 Nantes Cedex 03, France.
Comp Immunol Microbiol Infect Dis. 2010 Sep;33(5):375-87. doi: 10.1016/j.cimid.2009.01.001. Epub 2009 Mar 23.
Leptospirosis is a worldwide zoonosis. Today, serological diagnosis is generally assessed by MAT. We performed ELISA with a synthetic peptide derived from Hap1/lipL32 which is a protein expressed only by pathogenic Leptospira. Repeatability and thresholds were defined with 85 controls sera and 119 hospitalized leptospirosis. The PP-ELISA repeatability and IgM/IgG cut-off values were based on control sera. For these cut-off values, we observed the IgM-PP-ELISA specificity of 89%, whereas it was 100% for the IgG. Then, we compared PP-ELISA and standard MAT results for leptospirosis patients. The concordance rate for IgM-PP-ELISA and MAT was low (43%), whereas it was 85% for IgG-PP-ELISA and MAT. During the first 5 days after hospitalization, PP-ELISA gave positive results in 13 out of 16 patients (81%) whereas 8 out of 14 patients (57%) were positive to MAT. ELISA using Hap1/lipL 32-derived synthetic peptide PP is an earlier serological diagnosis of human leptospirosis than MAT.
钩端螺旋体病是一种世界性的人畜共患病。如今，血清学诊断通常通过 MAT 进行评估。我们使用源自仅由致病性钩端螺旋体表达的蛋白 Hap1/lipL32 的合成肽进行 ELISA。用 85 份对照血清和 119 份住院钩端螺旋体病血清来定义重复性和阈值。PP-ELISA 的重复性和 IgM/IgG 截断值基于对照血清确定。对于这些截断值，我们观察到 IgM-PP-ELISA 的特异性为 89%，而 IgG 的特异性为 100%。然后，我们比较了 PP-ELISA 和标准 MAT 对钩端螺旋体病患者的结果。IgM-PP-ELISA 和 MAT 的一致性率较低（43%），而 IgG-PP-ELISA 和 MAT 的一致性率为 85%。在住院后的前 5 天，PP-ELISA 在 16 名患者中的 13 名（81%）中产生阳性结果，而 MAT 在 14 名患者中的 8 名（57%）中产生阳性结果。使用源自 Hap1/lipL 32 的合成肽 PP 的 ELISA 是比 MAT 更早的人类钩端螺旋体病血清学诊断方法。
