Smith G N, Mickler E A, Myers S L, Brandt K D
Rheumatology Division, Department of Medicine, and Department of Anatomy, Indiana University School of Medicine, Room 545, 1110 W. Michigan Street, Indianapolis, IN 46202-5103, USA.
J Rheumatol. 2001 Jun;28(6):1341-6.
To determine how the quantity and molecular weight of synovial fluid hyaluronan (HA) within the synovial fluid (SF) of osteoarthritis (OA) joints is affected by intraarticular injection of HA.
Dogs in which OA was induced by transection of the anterior cruciate ligament received 5 weekly injections of HA (1.5 x 10(6) Da) in saline (10 mg/0.67 ml) or an equal volume of saline into the operated knee, beginning the day after surgery. Immediately before each injection, SF was aspirated and the volume of SF and the concentration of HA was measured (uronic acid), and the molecular weight of the HA in each sample was estimated by electrophoresis in agarose.
The volume of SF in the unstable knee increased after surgery, and the molecular weight decreased from approximately 2.5 x 10(6) Da to approximately 2 x 10(6) Da. Injection of HA did not affect the volume of SF or average molecular weight of HA in samples obtained immediately before each injection or at the end of the experiment, 12 weeks after surgery. The SF HA concentration fell from a baseline value of 2.3 +/- 0.1 mg/ml to 1.1 +/- 0.2 mg/ml the day after surgery and remained low throughout the course of injections. The HA concentration 12 weeks after surgery in the HA injected knees was approximately 40% lower than the preoperative value, although it increased slightly relative to saline injected knees (1.4 +/- 0.3 vs 1.1 +/- 0.01 mg/ml, respectively; p = 0.04).
Intraarticular injection of HA did not alter the volume of SF or molecular weight of HA in SF of OA canine knees, nor did it restore the HA concentration to that of normal canine SF.
确定关节内注射透明质酸（HA）如何影响骨关节炎（OA）关节滑液（SF）中透明质酸的数量和分子量。
通过切断前交叉韧带诱导患骨关节炎的犬，在手术后第二天开始，每周向手术膝关节内注射5次HA（1.5×10⁶Da）溶于生理盐水（10mg/0.67ml）或等体积生理盐水。每次注射前，抽取滑液，测量滑液体积和HA浓度（糖醛酸），并通过琼脂糖电泳估计每个样本中HA的分子量。
不稳定膝关节的滑液体积在手术后增加，分子量从约2.5×10⁶Da降至约2×10⁶Da。注射HA对每次注射前即刻或实验结束时（手术后12周）获得的样本中的滑液体积或HA平均分子量没有影响。滑液HA浓度从基线值2.3±0.1mg/ml在手术后一天降至1.1±0.2mg/ml，并在整个注射过程中保持较低水平。注射HA的膝关节在手术后12周时HA浓度比术前值低约40%，尽管相对于注射生理盐水的膝关节略有增加（分别为1.4±0.3与1.1±0.01mg/ml；p = 0.04）。
关节内注射HA并未改变OA犬膝关节滑液的体积或滑液中HA的分子量，也未将HA浓度恢复到正常犬滑液的水平。
