冻前生长速率和卵裂球数量对人类胚胎冷冻存活率及随后着床的影响。
The influence of prefreeze growth rate and blastomere number on cryosurvival and subsequent implantation of human embryos.
作者信息
Edgar D H, Jericho H, Bourne H, McBain J C
机构信息
Reproductive Biology Unit, Royal Women's Hospital, 132 Grattan Street, Carlton, Victoria 3053, Australia.
出版信息
J Assist Reprod Genet. 2001 Mar;18(3):135-8. doi: 10.1023/a:1009416205265.
PURPOSE
To determine whether the relatively low implantation rate of cryopreserved Day 2 embryos with only 2 blastomeres can be increased as a consequence of increasing their blastomere content by extending the prefreeze culture time.
METHODS
Of a total of 3480 Day 2 embryos studied, 1921 (55.2%) had reached the 4-cell stage by 40 h postinsemination (FAST) and were transferred or cryopreserved. The remaining embryos that underwent subsequent cell division by 46 h (INTERMEDIATE; 18.3% of total) or 66 h (SLOW; 20.3% of total) were also cryopreserved whereas the 6.2% that remained arrested at 66 h were discarded. Thawed embryos from each category were assessed for survival, post-thaw cleavage, and implantation.
RESULTS
The proportion of thawed embryos that survived, the proportion of surviving embryos that underwent post-thaw cleavage, and the implantation rate of transferred embryos were all reduced in the slower growing cryopreserved embryos.
CONCLUSIONS
The growth rate, and not the number of blastomeres per se, is a critical factor in predicting the developmental potential of cryopreserved embryos.
目的
通过延长冻存前的培养时间来增加仅含2个卵裂球的冷冻保存的第2天胚胎的卵裂球数量，从而确定其相对较低的着床率是否能够提高。
方法
在总共研究的3480个第2天胚胎中，1921个（55.2%）在授精后40小时（快速组）达到4细胞阶段，并进行了移植或冷冻保存。其余在46小时（中间组；占总数的18.3%）或66小时（慢速组；占总数的20.3%）进行后续细胞分裂的胚胎也进行了冷冻保存，而在66小时仍停滞的6.2%的胚胎则被丢弃。对来自每个类别的解冻胚胎进行存活、解冻后分裂和着床评估。
结果
生长较慢的冷冻保存胚胎中，解冻后存活的胚胎比例、存活胚胎解冻后分裂的比例以及移植胚胎的着床率均降低。
结论
生长速度而非卵裂球本身的数量是预测冷冻保存胚胎发育潜能的关键因素。
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