Oldstone M B, Lewicki H, Homann D, Nguyen C, Julien S, Gairin J E
Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
J Virol. 2001 Jul;75(14):6273-8. doi: 10.1128/JVI.75.14.6273-6278.2001.
Members of the Arenaviridae family have been isolated from mammalian hosts in disparate geographic locations, leading to their grouping as Old World types (i.e., lymphocytic choriomeningitis virus [LCMV], Lassa fever virus [LFV], Mopeia virus, and Mobala virus) and New World types (i.e., Junin, Machupo, Tacaribe, and Sabia viruses) (C. J. Peters, M. J. Buchmeier, P. E. Rollin, and T. G. Ksiazek, p. 1521-1551, in B. N. Fields, D. M. Knipe, and P. M. Howley [ed.], Fields virology, 3rd ed., 1996; P. J. Southern, p. 1505-1519, in B. N. Fields, D. M. Knipe, and P. M. Howley [ed.], Fields virology, 3rd ed., 1996). Several types in both groups-LFV, Junin, Machupo, and Sabia viruses-cause severe and often lethal human diseases. By sequence comparison, we noted that eight Old World and New World arenaviruses share several amino acids with the nucleoprotein (NP) that consists of amino acids (aa) 118 to 126 (NP 118-126) (RPQASGVYM) of LCMV that comprise the immunodominant cytotoxic T-lymphocyte (CTL) epitope for H-2(d) mice (32). This L(d)-restricted epitope constituted >97% of the total bulk CTLs produced in the specific antiviral or clonal responses of H-2(d) BALB mice. NP 118-126 of the Old World arenaviruses LFV, Mopeia virus, and LCMV and the New World arenavirus Sabia virus bound at high affinity to L(d). The primary H-2(d) CTL anti-LCMV response as well as that of a CTL clone responsive to LCMV NP 118-126 recognized target cells coated with NP 118-126 peptides derived from LCMV, LFV, and Mopeia virus but not Sabia virus, indicating that a common functional NP epitope exists among Old World arenaviruses. Use of site-specific amino acid exchanges in the NP CTL epitope among these arenaviruses identified amino acids involved in major histocompatibility complex binding and CTL recognition.
沙粒病毒科的成员已在不同地理位置的哺乳动物宿主中分离出来，因此被分为旧大陆型（即淋巴细胞性脉络丛脑膜炎病毒[LCMV]、拉沙热病毒[LFV]、莫佩亚病毒和莫巴拉病毒）和新大陆型（即胡宁病毒、马丘波病毒、塔卡里贝病毒和赛比亚病毒）（C.J.彼得斯、M.J.布赫迈尔、P.E.罗林和T.G.克夏泽克，第1521 - 1551页，载于B.N.菲尔兹、D.M.克尼普和P.M.豪利[编]，《菲尔兹病毒学》，第3版，1996年；P.J.萨瑟恩，第1505 - 1519页，载于B.N.菲尔兹、D.M.克尼普和P.M.豪利[编]，《菲尔兹病毒学》，第3版，1996年）。两组中的几种病毒——LFV、胡宁病毒、马丘波病毒和赛比亚病毒——可引发严重且往往致命的人类疾病。通过序列比较，我们注意到8种旧大陆和新大陆沙粒病毒与核蛋白（NP）共享若干氨基酸，这些氨基酸构成了LCMV的118至126位氨基酸（NP 118 - 126）（RPQASGVYM），该序列包含针对H - 2(d)小鼠的免疫显性细胞毒性T淋巴细胞（CTL）表位（32）。这个受L(d)限制的表位在H - 2(d) BALB小鼠的特异性抗病毒或克隆反应中产生的总CTL中占比超过97%。旧大陆沙粒病毒LFV、莫佩亚病毒和LCMV以及新大陆沙粒病毒赛比亚病毒的NP 118 - 126以高亲和力与L(d)结合。主要的H - 2(d) CTL抗LCMV反应以及对LCMV NP 118 - 126有反应的CTL克隆的反应识别涂有源自LCMV、LFV和莫佩亚病毒但不包括赛比亚病毒的NP 118 - 126肽的靶细胞，这表明旧大陆沙粒病毒之间存在共同的功能性NP表位。在这些沙粒病毒的NP CTL表位中使用位点特异性氨基酸交换确定了参与主要组织相容性复合体结合和CTL识别的氨基酸。
