Igarashi Y, Lee M Y, Matsuzaki S
Dokkyo University School of Medicine, Department of Biochemistry, Mibu Tochigi, Japan.
J Chromatogr B Biomed Sci Appl. 2001 Jun 15;757(2):269-76. doi: 10.1016/s0378-4347(01)00157-8.
The objective of the present study was to develop a specific method for the separation of tartrate-resistant acid phosphatase (TRAP) derived exclusively from osteoclasts. Heparin column-bound TRAP in human serum was separated into three peaks of TRAP activity when eluted with a linear gradient of sodium chloride. The last peak corresponded to TRAP 5b which was first named according to its electrophoretic mobility [Clin. Chem. 24 (1978) 309] and was considered to be derived from osteoclasts [J. Bone Miner. Res. 13 (1998) 683]. The second peak was found to be TRAP 5a. The height of the last peak varied from age to age.
