Scherthan H, Schönborn I
Abt. Humanbiologie & Humangenetik der Universität, Kaiserslautern, Germany.
Chromosome Res. 2001;9(4):273-82. doi: 10.1023/a:1016642528981.
Premeiotic and meiotic chromosome distribution was studied in rat testes suspensions by a triple-color fluorescent staining protocol which allows simultaneous visual inspection of two chromosomal targets highlighted by FISH together with immunostained SCP3 synaptonemal complex (SC) proteins which are marked by a third, composite color. Triple labeling with rat chromosome (RNO) 4q and 19p specific probes and SCP3 staining disclosed that homologs are separated in premeiotic and leptotene nuclei. Pairing of homologous chromosome regions commenced during early zygotene, with pairing of the small metacentric chromosomes 19 preceding that of the distal region of the long arm of RNO4. Our results show that homolog association occurs during zygotene of rat spermatogenesis, with small and large chromosomes showing a considerable asynchrony. Comparison with pairing progression in meiosis of other mammals suggests that asynchronous chromosome pairing reflects size differences within a complement.
通过三色荧光染色方案研究大鼠睾丸悬液中的减数分裂前和减数分裂染色体分布，该方案允许同时目视检查由荧光原位杂交（FISH）突出显示的两个染色体靶点，以及由第三种复合颜色标记的免疫染色的联会复合体（SC）蛋白SCP3。用大鼠染色体（RNO）4q和19p特异性探针进行三重标记以及SCP3染色显示，同源染色体在减数分裂前和细线期核中是分开的。同源染色体区域的配对在合线期早期开始，小的中着丝粒染色体19的配对先于RNO4长臂远端区域的配对。我们的结果表明，同源染色体联会发生在大鼠精子发生的合线期，小染色体和大染色体表现出相当大的不同步性。与其他哺乳动物减数分裂中配对进程的比较表明，异步染色体配对反映了一个染色体组内的大小差异。
