Manhart N, Vierlinger K, Spittler A, Bergmeister H, Sautner T, Roth E
Department of Surgical Research, University of Vienna, Vienna, Austria.
Ann Surg. 2001 Jul;234(1):92-7. doi: 10.1097/00000658-200107000-00014.
To determine the effect of oral glutamine feeding on lymphocyte subpopulations and glutathione metabolism in Peyer's patches (PPs) of healthy and endotoxemic mice.
Recent data indicate that nutrients both maintain nitrogen and energy balances and modulate cell and organ function. In particular, glutamine has an impact on gut and immune function. This is of special importance in the perioperative phase.
Female Balb/c mice were fed a glutamine-enriched diet or a control diet for 10 days. On day 7 25 microg lipopolysaccharide (LPS) or saline was injected. On day 3 after the challenge, mice were killed, total cell yield was determined, and lymphocyte subpopulations (total T cells, CD4+, CD8+ cells, and B cells) were analyzed by flow cytometry. One experimental group was treated with buthionine sulfoximine, a specific inhibitor of glutathione synthesis. The glutathione content in PPs was measured by high-performance liquid chromatography.
Glutamine administration led to a significant increase in total cell yield, including T and B cells, in PPs. The LPS-induced reduction of T cells (-45%) and of B cells (-30%) was significantly lower in glutamine-treated mice. Endotoxemia caused a 42% decrease of glutathione in control animals, but not in glutamine-treated animals. As with LPS, buthionine sulfoximine also lowered lymphocyte numbers and glutathione content of the PPs.
Administration of glutamine prevents LPS-stimulated lymphocyte atrophy in PPs, possibly by increasing the glutathione content in the PPs. Therefore, oral glutamine supply seems to be a suitable approach for improving intestinal immunity in immunocompromised patients.
确定口服谷氨酰胺对健康小鼠和内毒素血症小鼠派尔集合淋巴结（PPs）中淋巴细胞亚群及谷胱甘肽代谢的影响。
近期数据表明，营养物质既能维持氮和能量平衡，又能调节细胞和器官功能。特别是，谷氨酰胺对肠道和免疫功能有影响。这在围手术期尤为重要。
雌性Balb/c小鼠分别喂食富含谷氨酰胺的饮食或对照饮食10天。在第7天，注射25微克脂多糖（LPS）或生理盐水。在激发后第3天，处死小鼠，测定总细胞产量，并通过流式细胞术分析淋巴细胞亚群（总T细胞、CD4⁺、CD8⁺细胞和B细胞）。一个实验组用谷胱甘肽合成的特异性抑制剂丁硫氨酸亚砜胺处理。通过高效液相色谱法测量PPs中的谷胱甘肽含量。
给予谷氨酰胺导致PPs中总细胞产量显著增加，包括T细胞和B细胞。在谷氨酰胺处理的小鼠中，LPS诱导的T细胞减少（-45%）和B细胞减少（-30%）明显更低。内毒素血症导致对照动物的谷胱甘肽减少42%，但在谷氨酰胺处理的动物中未出现这种情况。与LPS一样，丁硫氨酸亚砜胺也降低了PPs中的淋巴细胞数量和谷胱甘肽含量。
给予谷氨酰胺可能通过增加PPs中的谷胱甘肽含量来防止LPS刺激的PPs中淋巴细胞萎缩。因此，口服谷氨酰胺供应似乎是改善免疫功能低下患者肠道免疫力的合适方法。
