Fan Jun, Xie Yong, Li Xuedong, Guo Guanghua, Meng Qingyan, Xiu Yiping, Li Tairan, Feng Wei, Ma Liang
Department of Burns, Northern Hospital, 83 Wenhua Road, Shenyang 110015, Liaoning, China.
Burns. 2009 Aug;35(5):687-94. doi: 10.1016/j.burns.2008.10.013. Epub 2009 Mar 9.
The aim of this study was to investigate the influence of apoptosis on Peyer's patches and the intestinal immunoglobulin A (IgA) response in burned mice. Sixty male Balb/c mice were randomly assigned into the sham-burn (control) group (n=30) and the burn group (n=30). The mice in the burn group received a full-thickness scald burn over 20% of the total body surface area (TBSA), on the back. At 12, 24 and 72 h, respectively, after injury, the burned mice (n=10, at every time point) were anaesthetised and their entire intestines were collected. The mice in the sham-burn group were treated with the same procedure as above, except for the burn injury. The number of Peyer's patches on every entire intestine and the total Peyer's patches cell yield were counted. The changes of lymphocyte subpopulations in Peyer's patches were measured by flow cytometry (FCM). And the levels of intestinal IgA were examined by enzyme-linked immunosorbent assay (ELISA). Fluoresceinisothiocyanate (FITC)-conjugated Annexin-tau and propidium iodide (PI) double-staining cells were analysed by FCM for apoptotic ratio in Peyer's patches. The results showed that the total Peyer's patch cell yield and the numbers of CD3, CD4, CD8 and CD19 cells were significantly decreased at 12, 24 and 72 h after injury (P<0.05), and that the intestinal IgA levels were markedly reduced at 24 and 72 h (P<0.05). On the other hand, total apoptotic ratio and all cell subpopulation apoptosis in Peyer's patches were dramatically increased at 12, 24 and 72 h after injury (P<0.05). These results indicated that severe burns led to a significant decrease in the number of Peyer's patch cells and in intestinal IgA levels, which was closely associated with strongly increased apoptosis in Peyer's patches.
本研究旨在探讨细胞凋亡对烧伤小鼠派尔集合淋巴结及肠道免疫球蛋白A（IgA）应答的影响。将60只雄性Balb/c小鼠随机分为假烧伤（对照）组（n = 30）和烧伤组（n = 30）。烧伤组小鼠背部接受占全身表面积（TBSA）20%的全层烫伤。分别在伤后12、24和72小时，将烧伤小鼠（每个时间点n = 10）麻醉并收集其整个肠道。假烧伤组小鼠除不进行烧伤损伤外，接受与上述相同的处理。计数每段完整肠道上派尔集合淋巴结的数量及派尔集合淋巴结细胞总产量。采用流式细胞术（FCM）检测派尔集合淋巴结中淋巴细胞亚群的变化。并通过酶联免疫吸附测定（ELISA）检测肠道IgA水平。采用FCM分析异硫氰酸荧光素（FITC）偶联膜联蛋白V和碘化丙啶（PI）双染细胞，以检测派尔集合淋巴结中的细胞凋亡率。结果显示，伤后12、24和72小时，派尔集合淋巴结细胞总产量以及CD3、CD4、CD8和CD19细胞数量显著减少（P < 0.05），且肠道IgA水平在24和72小时显著降低（P < 0.05）。另一方面，伤后12、24和72小时，派尔集合淋巴结中的总凋亡率及所有细胞亚群凋亡均显著增加（P < 0.05）。这些结果表明，严重烧伤导致派尔集合淋巴结细胞数量及肠道IgA水平显著降低，这与派尔集合淋巴结中凋亡的强烈增加密切相关。
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