盘基网柄菌发育过程中参与环磷酸腺苷应答的基因的空间表达模式。

Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium discoideum development.

作者信息

Tsujioka M, Yokoyama M, Nishio K, Kuwayama H, Morio T, Katoh M, Urushihara H, Saito T, Ochiai H, Tanaka Y, Takeuchi I, Maeda M

机构信息

Department of Biology, Graduate School of Science, Osaka University, Machikaneyama-cho 1-16, Toyonaka, Osaka 560-0043, Japan.

出版信息

Dev Growth Differ. 2001 Jun;43(3):275-83. doi: 10.1046/j.1440-169x.2001.00572.x.

DOI:10.1046/j.1440-169x.2001.00572.x

PMID:11422293

Abstract

The spatial expression patterns of genes involved in cyclic adenosine monophosphate (cAMP) responses during morphogenesis in Dictyostelium discoideum were analyzed by in situ hybridization. Genes encoding adenylyl cyclase A (ACA), cAMP receptor 1, G-protein alpha2 and beta subunits, cytosolic activator of ACA (CRAC and Aimless), catalytic subunit of protein kinase A (PKA-C) and cAMP phosphodiesterases (PDE and REG-A) were preferentially expressed in the anterior prestalk (tip) region of slugs, which acts as an organizing center. MAP kinase ERK2 (extracellular signal-regulated kinase-2) mRNA, however, was enriched in the posterior prespore region. At the culmination stage, the expression of ACA, CRAC and PKA-C mRNA increased in prespore cells in contrast with the previous stage. However, no alteration in the site of expression was observed for the other mRNA analyzed. Based on these findings, two and four classes of expression patterns were catalogued for these genes during the slug and culmination stages, respectively. Promoter analyses of genes in particular classes should enhance understanding of the regulation of dynamic and coordinated gene expression during morphogenesis.

摘要

通过原位杂交分析了盘基网柄菌形态发生过程中参与环磷酸腺苷（cAMP）反应的基因的空间表达模式。编码腺苷酸环化酶A（ACA）、cAMP受体1、G蛋白α2和β亚基、ACA的胞质激活剂（CRAC和Aimless）、蛋白激酶A的催化亚基（PKA-C）和cAMP磷酸二酯酶（PDE和REG-A）的基因在蛞蝓的前柄细胞（顶端）区域优先表达，该区域起着组织中心的作用。然而，MAP激酶ERK2（细胞外信号调节激酶2）mRNA在孢子后区域富集。在发育成熟阶段，与前一阶段相比，ACA、CRAC和PKA-C mRNA在孢子细胞中的表达增加。然而，对于分析的其他mRNA，未观察到表达位点的改变。基于这些发现，分别在蛞蝓和发育成熟阶段为这些基因编目了两类和四类表达模式。对特定类别的基因进行启动子分析应能增强对形态发生过程中动态和协调基因表达调控的理解。