沙漠蝗（Schistocerca gregaria）中两种不同神经肽激素原的cDNA克隆及转录本分布

cDNA cloning and transcript distribution of two different neuroparsin precursors in the desert locust, Schistocerca gregaria.

作者信息

Janssen T, Claeys I, Simonet G, De Loof A, Girardie J, Vanden Broeck J

机构信息

Laboratory for Developmental Physiology and Molecular Biology, Zoological Institute, Naamsestraat 59, B-3000 K.U. Leuven, Belgium.

出版信息

Insect Mol Biol. 2001 Apr;10(2):183-9. doi: 10.1046/j.1365-2583.2001.00257.x.

DOI:10.1046/j.1365-2583.2001.00257.x

PMID:11422514

Abstract

Neuroparsins were originally identified in locust corpus cardiacum extracts as folliculostatic or 'antigonadotropic' neuropeptides. This paper presents the cloning of two different neuroparsin precursor cDNAs from the brain of the desert locust, Schistocerca gregaria. The first transcript encodes the precursor (Scg-NPP1) of S. gregaria neuroparsin A and B, whereas the second codes for a novel neuroparsin-related peptide precursor (Scg-NPP2). Both precursors display significant sequence similarities with each other and with the Locusta migratoria neuroparsin (Lom-NPP) and Aedes aegypti ovary ecdysteroidogenic hormone (Aea-OEH1) precursors. Northern blot analysis revealed that these neuroparsin transcripts are present in larval and adult locust brains. Interestingly, the Scg-NPP2 mRNA content proved to be strongly regulated during the reproductive cycle in both adult males and females.

摘要

神经素最初是在蝗虫心侧体提取物中作为卵泡静止或“抗促性腺”神经肽被鉴定出来的。本文展示了从沙漠蝗虫（沙漠飞蝗）大脑中克隆出的两种不同的神经素前体cDNA。第一个转录本编码沙漠飞蝗神经素A和B的前体（Scg-NPP1），而第二个编码一种新的神经素相关肽前体（Scg-NPP2）。这两种前体彼此之间以及与飞蝗神经素（Lom-NPP）和埃及伊蚊卵巢蜕皮甾体生成激素（Aea-OEH1）前体都显示出显著的序列相似性。Northern印迹分析表明，这些神经素转录本存在于幼虫和成虫的蝗虫大脑中。有趣的是，事实证明，Scg-NPP2 mRNA的含量在成年雄性和雌性的生殖周期中受到强烈调控。