在日本人群中未发现神经肽Y信号肽中亮氨酸（7）到脯氨酸（7）的多态性。

A leucine(7)-to-proline(7) polymorphism in the signal peptide of neuropeptide Y was not identified in the Japanese population.

作者信息

Makino K, Kataoka Y, Hirakawa Y, Ikeda A, Yamauchi A, Oishi A R

机构信息

Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan.

出版信息

J Clin Pharm Ther. 2001 Jun;26(3):201-3. doi: 10.1046/j.1365-2710.2001.00346.x.

DOI:10.1046/j.1365-2710.2001.00346.x

PMID:11422604

Abstract

OBJECTIVE

A Leu(7)-to-Pro(7) polymorphism exists in the signal peptide part of prepro-neuropeptide Y (NPY) and this polymorphism is associated with high serum cholesterol and LDL cholesterol levels in both Finnish and Dutch people. There was a significant difference in the allelic frequencies among populations with different genetic backgrounds. Therefore, the present study was designed to test whether Japanese people had the Leu(7)-to-Pro(7) polymorphism in the NPY signal peptide.

METHODS

A total of 102 healthy Japanese volunteers supplied blood samples, from which leucocyte DNA was isolated. Primer pairs for the polymerase chain reaction (PCR) were made according to Karvonen et al. Direct sequencing of the PCR product was carried out by the dye terminator method using a capillary electrophoresis sequencer.

RESULTS

All 102 young healthy Japanese subjects showed Leu(7)/Leu(7) but not Leu(7)/Pro(7) or Pro(7)/Pro(7) in the NPY signal peptide.

CONCLUSION

The incidence of the Leu(7)-to-Pro(7) polymorphism in the signal peptide of NPY was extremely low in Japanese people, suggesting that the polymorphism producing Pro(7) is not one of the genetic determinant factors affecting serum cholesterol levels in Japanese people.

摘要