Danielsson B, Mattiasson B, Karlsson R, Winqvist F
Biotechnol Bioeng. 1979 Oct;21(10):1749-66. doi: 10.1002/bit.260211005.
The enzyme thermistor measures the heat produced by the action of an immobilized enzyme on a substrate present in the sample. Its application in analysis of discrete samples, e.g., in clinical chemistry, is well documented, but it has not been used so far for continuous measurements. We decribe here the application of the enzyme thermistor for continuous monitoring and control of enzyme reactors. An enzyme thermistor filled with coimmobilized glucose oxidase and catalase was used to measure the amount of glucose in the outflow from a column reactor containing immobilized lactase acting on a lactose solution pumped through the reactor. The lactose conversion was kept on a constant level, irrespective of the actual enzymatic activity in the reactor, by regulating the flow through the reactor. The experiments were carried out with aqueous solutions of lactose as well as with whey from cow's milk.
酶热敏电阻可测量固定化酶作用于样品中存在的底物时产生的热量。其在离散样品分析中的应用，如在临床化学中，已有充分记录，但迄今为止尚未用于连续测量。我们在此描述酶热敏电阻在酶反应器连续监测和控制中的应用。使用填充有共固定化葡萄糖氧化酶和过氧化氢酶的酶热敏电阻来测量来自柱式反应器流出物中的葡萄糖量，该柱式反应器含有固定化乳糖酶，作用于泵送通过反应器的乳糖溶液。通过调节流经反应器的流量，乳糖转化率可保持在恒定水平，而与反应器中的实际酶活性无关。实验使用乳糖水溶液以及牛奶中的乳清进行。
