Foa C
Bull Cancer. 1979;66(3):235-58.
The relationships between differentiation and malignant transformation were studied in human malignant melanomas in vivo and in vitro. Melanocyte differentiation was assessed by ultrastructural morphological characteristis (the appearance of the melanosomes and related structures) localization of dopa-oxidase and assay of 5-S-cysteinyldopa, a specific metabolite. The transformed characteristic of the cells in vitro was evaluated by their ability to give rise to established cell lines, karyological modifications and heterotransplantation in Nude mice and Syrian hamsters. Morphological variability of the cells in malignant melanomas is accompanied by variability in the localization of dopa-oxidase, the level of 5-S-cysteinyldopa, chromosome pattern and their heterotransplantibility. The lack of pigmentation in some malignant melanoma lines can result from either an irreversible loss of some functions which give rise in melanization and the malignancy in maintained, or by phenomenon of regulation determined by intra or extra-cellular factors with the loss of heterotransplantability. Modulation phenomena affecting tumorigenicity and pigmentation although sometimes concomitant are not identical.
在体内和体外对人类恶性黑色素瘤中分化与恶性转化之间的关系进行了研究。通过超微结构形态特征（黑素小体及相关结构的外观）、多巴氧化酶的定位以及特定代谢产物5-S-半胱氨酰多巴的检测来评估黑素细胞分化。通过细胞产生已建立细胞系的能力、核型改变以及在裸鼠和叙利亚仓鼠体内的异种移植来评估体外细胞的转化特征。恶性黑色素瘤细胞的形态变异性伴随着多巴氧化酶定位、5-S-半胱氨酰多巴水平、染色体模式及其异种移植能力的变异性。一些恶性黑色素瘤细胞系缺乏色素沉着可能是由于导致黑色素化的某些功能不可逆转地丧失，而恶性程度得以维持，或者是由细胞内或细胞外因素决定的调节现象导致异种移植能力丧失。影响致瘤性和色素沉着的调节现象虽然有时同时出现，但并不相同。
