Smith L M, Priest J W, Lammie P J, Mead J R
Department of Pediatrics, Emory University and Atlanta Veterans Medical Center, Decatur, Georgia 30033, USA.
J Parasitol. 2001 Jun;87(3):704-7. doi: 10.1645/0022-3395(2001)087[0704:HTABCI]2.0.CO;2.
Cryptosporidial infection in humans results in parasite-specific IgG, IgM, and IgA antibody responses, but little is known of the cell-mediated immune responses to cryptosporidial antigens. In a convenience sample of 35 Haitian residents, there was a high level of cryptosporidial exposure (>90%) as determined by immunoblot reactivity of serum against cryptosporidial antigens. An attempt was made to determine if there was a relationship between antibody and T cell-mediated responses to recombinant Cp23 antigen and how this correlated with reactivity to crude sporozoite antigen preparations (SAg). T cell reactivity was greater against SAg (57%) than to Cp23 (34.3%) as measured by [3H]thymidine incorporation. Proliferative responses to Cp23 were significantly correlated with SAg responses. By enzyme-linked immunosorbent assay, most persons had IgG responses to both SAg (91.4%) and to recombinant Cp23 (88.5%). Antibody responses were greater among persons who exhibited T cell responses to SAg and Cp23. This study demonstrates that recombinant Cp23 antigen could be a useful antigen for detection of both antibody and cell-mediated responses in epidemiologic studies.
人类隐孢子虫感染会引发针对寄生虫的特异性IgG、IgM和IgA抗体反应，但对于隐孢子虫抗原的细胞介导免疫反应却知之甚少。在一个由35名海地居民组成的便利样本中，通过血清对隐孢子虫抗原的免疫印迹反应性测定，发现隐孢子虫暴露水平很高（>90%）。研究试图确定抗体与T细胞介导的对重组Cp23抗原的反应之间是否存在关联，以及这与对粗制子孢子抗原制剂（SAg）的反应性如何相关。通过[3H]胸腺嘧啶核苷掺入法测定，T细胞对SAg的反应性（57%）高于对Cp23的反应性（34.3%）。对Cp23的增殖反应与对SAg的反应显著相关。通过酶联免疫吸附测定法，大多数人对SAg（91.4%）和重组Cp23（88.5%）都有IgG反应。在对SAg和Cp23表现出T细胞反应的人群中，抗体反应更强。这项研究表明，重组Cp23抗原在流行病学研究中可能是一种用于检测抗体和细胞介导反应的有用抗原。
