Auwanit W, Mukai T, Ayuthaya P I, Kurata T, Ikuta K
Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand.
AIDS Res Hum Retroviruses. 2001 Jun 10;17(9):867-71. doi: 10.1089/088922201750252070.
We isolated two CRF01_AE human immunodeficiency virus type 1 (95TNIH022 and 95TNIH047) from the 1995 blood samples derived from asymptomatic carriers in Ubonratchatani province of northeastern Thailand. Both isolates can replicate in peripheral blood mononuclear cells, but not in several T cell lines examined. The full-length sequences recovered from proviruses in infected cells by long-range polymerase chain reaction were determined. Phylogenetic analyses of these sequences at individual genes showed them to be closely related to those of reported CRF01_AE HIV-1, such as 1990 isolate CM240 and 1993 isolate 93TH253. Two isolates in this study also showed a similar pattern of CRF01_AE mosaicism and a similar structure at the long terminal repeat, i.e., a copy number of NF-kappaB binding sites, sequence at the TATA box, and the putative secondary structure of stem-loop in the transactivation response region. Our results showed that 1995 Thai E isolates could contribute to our understanding of the epidemiology, pathogenesis, and diagnostics of HIV-1 CRF01_AE and further to vaccine development.
我们从1995年泰国东北部乌汶叻差他尼省无症状携带者的血液样本中分离出两株CRF01_AE 1型人类免疫缺陷病毒（95TNIH022和95TNIH047）。两株分离株均可在外周血单核细胞中复制，但在所检测的几种T细胞系中不能复制。通过长距离聚合酶链反应从感染细胞中的前病毒中获得全长序列并进行测定。对这些序列的单个基因进行系统发育分析，结果显示它们与已报道的CRF01_AE HIV-1密切相关，如1990年分离株CM240和1993年分离株93TH253。本研究中的两株分离株在CRF01_AE镶嵌模式以及长末端重复序列结构方面也表现出相似性，即核因子κB结合位点的拷贝数、TATA盒处的序列以及反式激活应答区域中茎环的假定二级结构。我们的结果表明，1995年泰国E分离株有助于我们了解HIV-1 CRF01_AE的流行病学、发病机制和诊断，进而有助于疫苗开发。
