未标记肽与标记蛋白复合体系中同核三键J(H(N)Hα)耦合常数的测量：应用于与丙型肝炎病毒（HCV）NS3蛋白蛋白酶结构域结合的十肽抑制剂
Measurement of homonuclear three-bond J(H(N)Halpha) coupling constants in unlabeled peptides complexed with labeled proteins: application to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of hepatitis C virus (HCV).
作者信息
Cicero D O, Barbato G, Koch U, Ingallinella P, Bianchi E, Sambucini S, Neddermann P, De Francesco R, Pessi A, Bazzo R
机构信息
IRBM P. Angeletti, Pomezia (Rome), Italy.
出版信息
J Biomol NMR. 2001 May;20(1):23-9. doi: 10.1023/a:1011281527761.
A new isotope-filtered experiment has been designed to measure homonuclear three-bond J(H(N)Halpha) coupling constants of unlabeled peptides complexed with labeled proteins. The new experiment is based on the 3D HNHA pulse scheme, and belongs to the 'quantitative J-correlation' type. It has been applied to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of human hepatitis C virus (HCV).
已设计出一种新的同位素过滤实验，用于测量与标记蛋白复合的未标记肽的同核三键J(H(N)Hα)耦合常数。新实验基于3D HNHA脉冲序列，属于“定量J相关”类型。它已应用于与人类丙型肝炎病毒（HCV）NS3蛋白的蛋白酶结构域结合的十肽抑制剂。
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