上皮癌细胞迁移：趋化因子受体的作用？

Epithelial cancer cell migration: a role for chemokine receptors?

作者信息

Scotton C J, Wilson J L, Milliken D, Stamp G, Balkwill F R

机构信息

Imperial Cancer Research Fund Translational Oncology Laboratory, Barts and the Royal London School of Medicine and Dentistry, London EC1M 6BQ, United Kingdom.

出版信息

Cancer Res. 2001 Jul 1;61(13):4961-5.

PMID:11431324

Abstract

We investigated the possibility that chemokine gradients influence migration of human ovarian epithelial tumor cells. Of 14 chemokine receptors investigated, only CXCR4 was expressed on ovarian cancer cells. CXCR4 mRNA localized to a subpopulation of tumor cells in ovarian cancer biopsies. Ovarian cancer cell lines and cells freshly isolated from ascites expressed CXCR4 protein. The CXCR4 ligand, CXCL12, was found in ascites from 63 patients. CXCL12 elicited intracellular calcium flux and directed migration and changes in integrin expression in ovarian cancer cells. CXCR4 may influence cell migration in the peritoneum, a major route for ovarian cancer spread, and could be a therapeutic target.

摘要

我们研究了趋化因子梯度影响人卵巢上皮肿瘤细胞迁移的可能性。在所研究的14种趋化因子受体中，只有CXCR4在卵巢癌细胞上表达。CXCR4 mRNA定位于卵巢癌活检组织中的一部分肿瘤细胞。卵巢癌细胞系和从腹水中新鲜分离的细胞表达CXCR4蛋白。在63例患者的腹水中发现了CXCR4配体CXCL12。CXCL12可引起卵巢癌细胞内钙流，指导细胞迁移并改变整合素表达。CXCR4可能影响卵巢癌主要扩散途径——腹膜中的细胞迁移，并且可能成为一个治疗靶点。