Fu Y, Niu D, Ruan H, Yu X P, Chen G, He G Q, Yang P X
Animal Science College of Zhejiang University, Hangzhou 310029, China.
Yi Chuan Xue Bao. 2001;28(6):502-9.
The very sensitive quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) was used to investigate the expression of mRNA for the inhibin alpha and inhibin/activin beta A subunit in the developing ovarian follicles of the duck. The results indicated all follicles showed the expression of mRNA for the inhibin alpha and inhibin/activin beta A. The inhibin alpha subunit mRNA is expressed more abundantly than the beta A subunit in the large preovulatory follicles. Competitive RT-PCR revealed that the expression of inhibin alpha subunit is the highest in small yellow follicles (SYF), the mean relative content for the F1, F2, F3, F4/5 and LWF (large white follicle) was 0.26 +/- 0.05, 0.28 +/- 0.07, 0.57 +/- 0.12, 0.98 +/- 0.09 and 0.026 +/- 0.006, respectively, compared to a mean relative content of 1.00 for the SYF. The highest level of inhibin/activin beta A mRNA was found in the F1 follicle, the mean relative content for the F2, F3, F4/5, SYF and LWF was 0.218 +/- 0.09, 0.111 +/- 0.03, 0.058 +/- 0.011, 0.053 +/- 0.013 and 0.005 +/- 0.002, respectively, compared to a mean relative content of 1.00 for the F1 follicle. Our data suggest that the expression of the alpha subunit is reduced with follicular development whereas beta A subunit expression is dramatically enhanced, which indicates the expression of inhibin alpha and inhibin/activin beta A subunit is differentially regulated during follicular development. In addition, the highest level of beta A mRNA in F1 follicle indicates the production of dimeric inhibin and/or activin primarily occurred in the largest F1 follicle.
采用高灵敏度的定量竞争性逆转录-聚合酶链反应（RT-PCR），研究鸭发育中的卵泡中抑制素α和抑制素/激活素βA亚基的mRNA表达。结果表明，所有卵泡均显示出抑制素α和抑制素/激活素βA的mRNA表达。在大的排卵前卵泡中，抑制素α亚基mRNA的表达比βA亚基更丰富。竞争性RT-PCR显示，抑制素α亚基在小黄卵泡（SYF）中表达最高，F1、F2、F3、F4/5和大白卵泡（LWF）的平均相对含量分别为0.26±0.05、0.28±0.07、0.57±0.12、0.98±0.09和0.026±0.006，而SYF的平均相对含量为1.00。抑制素/激活素βA mRNA的最高水平出现在F1卵泡中，F2、F3、F4/5、SYF和LWF的平均相对含量分别为0.218±0.09、0.111±0.03、0.058±0.011、0.053±0.013和0.005±0.002，而F1卵泡的平均相对含量为1.00。我们的数据表明，随着卵泡发育，α亚基的表达降低，而βA亚基的表达显著增强，这表明抑制素α和抑制素/激活素βA亚基在卵泡发育过程中受到不同的调控。此外，F1卵泡中βA mRNA的最高水平表明二聚体抑制素和/或激活素的产生主要发生在最大的F1卵泡中。
