碱性成纤维细胞生长因子增强了在基于非织造透明质酸的聚合物支架上生长的大鼠骨髓基质细胞的体外矿化作用。

Basic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold.

作者信息

Lisignoli G, Zini N, Remiddi G, Piacentini A, Puggioli A, Trimarchi C, Fini M, Maraldi N M, Facchini A

机构信息

Laboratorio di Immunologia e Genetica. Istituti Ortopedici Rizzoli, Bologna, Italy.

出版信息

Biomaterials. 2001 Aug;22(15):2095-105. doi: 10.1016/s0142-9612(00)00398-7.

DOI:10.1016/s0142-9612(00)00398-7

PMID:11432589

Abstract

A biodegradable non-woven hyaluronic acid polymer scaffold (Hyaff 11) was analysed in vitro as a carrier vehicle for differentiation and mineralization of rat bone marrow stromal cells (BMSC). BMSC were grown on Hyaff 11 in a mineralizing medium in the presence/absence of basic fibroblast growth factor (bFGF). Osteoblastic differentiation was investigated by light and electron microscopy analysing the expression of osteogenic markers: calcium, alkaline phosphatase (AP), osteopontin (OP), bone sialoprotein (BSP) and collagen type 1. We also measured proliferation, AP activity and mRNA expression of AP and osteocalcin (OC). Electron microscopy and Toluidine-blue staining demonstrated that bFGF accelerated (day 20 vs. day 40) and increased mineralization. With bFGF, calcium, OP and BSP were strongly enhanced at day 40, whereas AP decreased. Our in vitro results demonstrate that Hyaff 11 is a useful vehicle for growth, differentiation and mineralization of rat BMSC, and that it permits bone development.

摘要

一种可生物降解的非织造透明质酸聚合物支架（Hyaff 11）在体外被分析作为大鼠骨髓基质细胞（BMSC）分化和矿化的载体。BMSC在有/无碱性成纤维细胞生长因子（bFGF）的矿化培养基中在Hyaff 11上生长。通过光学和电子显微镜分析成骨标志物钙、碱性磷酸酶（AP）、骨桥蛋白（OP）、骨唾液蛋白（BSP）和I型胶原的表达来研究成骨细胞分化。我们还测量了AP和骨钙素（OC）的增殖、AP活性和mRNA表达。电子显微镜和甲苯胺蓝染色表明bFGF加速了（第20天与第40天相比）并增加了矿化。使用bFGF时，第40天时钙、OP和BSP显著增强，而AP降低。我们的体外结果表明，Hyaff 11是大鼠BMSC生长、分化和矿化的有用载体，并且它促进骨发育。