Parekh M H, Lobel R, O'Connor L J, Leggett R E, Levin R M
Department of Uro-Gynecology, Albany Medical College, New York, USA.
J Urol. 2001 Jul;166(1):341-6.
There is increasing evidence that ischemia/reperfusion is a major etiological factor in the progression of bladder dysfunction after partial outlet obstruction. If this evidence is correct, treatment with an antioxidant should be beneficial in rabbits subjected to partial outlet obstruction. We designed the current study to determine if diets high in alpha-tocopherol protected the rabbit bladder against dysfunction induced by partial outlet obstruction.
A total of 32 rabbits were separated into 4 groups of 8. Groups 1 and 2 were placed on a diet enriched with 1,000 IU/kg. alpha-tocopherol, and groups 3 and 4 were fed a regular diet containing 44 IU/kg. alpha-tocopherol. After 4 weeks partial outlet obstruction was created in groups 1 and 3, while groups 2 and 4 underwent sham operation. After 4 weeks of obstruction the rabbits were anesthetized and the bladders were rapidly excised. Four longitudinal strips obtained from the bladder body were used for contractility studies. The balance of the bladder body was separated between muscle and mucosa. Each section was frozen and stored at -70C for analysis of malondialdehyde as a measure of peroxidation and for alpha-tocopherol concentrations.
Feeding rabbits a diet high in alpha-tocopherol resulted in significant protection against the development of contractile dysfunction after partial outlet obstruction. The protective effect of alpha-tocopherol was related to significantly decreased malondialdehyde and significantly increased tissue concentrations of alpha-tocopherol.
These data indicate that a major etiology of bladder dysfunction secondary to partial outlet obstruction is related to free radical generation and resultant membrane lipid peroxidation.
越来越多的证据表明，缺血/再灌注是部分出口梗阻后膀胱功能障碍进展的主要病因。如果这一证据正确，那么用抗氧化剂治疗对部分出口梗阻的兔子应该有益。我们设计了本研究以确定富含α-生育酚的饮食是否能保护兔膀胱免受部分出口梗阻诱导的功能障碍。
总共32只兔子被分成4组，每组8只。第1组和第2组给予富含1000 IU/kgα-生育酚的饮食，第3组和第4组给予含44 IU/kgα-生育酚的常规饮食。4周后，第1组和第3组进行部分出口梗阻，而第2组和第4组进行假手术。梗阻4周后，将兔子麻醉并迅速切除膀胱。从膀胱体获取的4条纵向条带用于收缩性研究。膀胱体的其余部分分离为肌肉和黏膜。每个部分冷冻并储存在-70°C，用于分析丙二醛以衡量过氧化程度以及α-生育酚浓度。
给兔子喂食富含α-生育酚的饮食可显著保护其免受部分出口梗阻后收缩功能障碍的发展。α-生育酚的保护作用与丙二醛显著降低以及组织中α-生育酚浓度显著升高有关。
这些数据表明，部分出口梗阻继发膀胱功能障碍的主要病因与自由基生成及由此导致的膜脂质过氧化有关。
