确定食用动物组织中磺胺二甲嘧啶常规残留监测的程序。

Determining the procedure for routine residue monitoring of sulfamethazine in edible animal tissues.

作者信息

Furusawa N

机构信息

Faculty of Human Life Science, Osaka City University, Sumiyoshi-ku, Osaka, Japan.

出版信息

Biomed Chromatogr. 2001 Jun;15(4):235-9. doi: 10.1002/bmc.48.

DOI:10.1002/bmc.48

PMID:11438963

Abstract

A simplified method to determine/identify residual sulfamethazine (SMZ) in edible tissues from cattle, pigs, chickens and sheep by a high-performance liquid chromatography (HPLC) with a photo-diode array detector is presented. The sample preparation was performed by homogenizing with 30% (v/v) ethanol in water followed by an Ultrafree-MC/PL as a centrifugal ultra-filtration unit. For determination/identification of SMZ, a reversed-phase C(4) column and a mobile phase of 15% (v/v) ethanol in water with a photo-diode array detector was used. Average recoveries from spiked SMZ (0.1-1.0 mg kg(-1)) were >or=80% with coefficients of variation between 1.3 and 4.3%. The limits of quantitation were calculated to be 0.057-0.060 mg kg(-1). The total time and solvent required for the analysis of one sample were <40 min and <2 mL of ethanol, respectively.

摘要

本文介绍了一种通过高效液相色谱（HPLC）结合光电二极管阵列检测器来测定/鉴定牛、猪、鸡和羊可食用组织中残留磺胺二甲嘧啶（SMZ）的简化方法。样品制备方法为：先用30%（v/v）乙醇水溶液匀浆，然后使用Ultrafree-MC/PL作为离心超滤装置。测定/鉴定SMZ时，使用反相C(4)柱，流动相为15%（v/v）乙醇水溶液，并配备光电二极管阵列检测器。加标SMZ（0.1 - 1.0 mg kg(-1)）的平均回收率≥80%，变异系数在1.3%至4.3%之间。定量限经计算为0.057 - 0.060 mg kg(-1)。分析一个样品所需的总时间和溶剂分别小于40分钟和2毫升乙醇。