Furusawa N
Graduate School of Human Life Science, Osaka City University, Osaka, Japan.
J Chromatogr Sci. 2002 Aug;40(7):355-8. doi: 10.1093/chromsci/40.7.355.
A simplified determining method for the routine monitoring of residual amprolium in edible chicken tissues (muscle and liver) is developed using a high-performance liquid chromatographic (HPLC) method with a photodiode-array detector after sample cleanup by an Ultrafree-MC/PL centrifugal ultrafiltration unit. For the HPLC determination and identification, a Mightysil RP-4 GP column and a mobile phase of an ethanol-5 mM 1-heptanesulfonic acid sodium salt solution (35:65, v/v) using an ion-pairing system with a photodiode-array detector are used. Average recoveries (spiked at 0.3-3.0 microg/g) are > 90%. The inter- and intravariabilities are 1.9-2.4%. The limits of quantitation are 0.22 microg/g for muscle and 0.25 microg/g for liver. The total time and solvent required for the analysis of one sample are < 20 min and < 2 mL of ethanol, respectively. No toxic solvents and regents are used.
开发了一种用于常规监测食用鸡肉组织（肌肉和肝脏）中氨丙啉残留量的简化测定方法，该方法采用高效液相色谱（HPLC）法，配备光电二极管阵列检测器，样品经Ultrafree-MC/PL离心超滤装置净化后进行分析。对于HPLC测定和鉴定，使用Mightysil RP-4 GP柱和乙醇-5 mM 1-庚烷磺酸钠溶液（35:65，v/v）的流动相，采用离子对系统和光电二极管阵列检测器。平均回收率（加标量为0.3 - 3.0 μg/g）> 90%。批间和批内变异系数为1.9 - 2.4%。肌肉的定量限为0.22 μg/g，肝脏的定量限为0.25 μg/g。分析一个样品所需的总时间和溶剂量分别< 20分钟和< 2 mL乙醇。不使用有毒溶剂和试剂。
