肝素对鼠伤寒沙门氏菌脂多糖刺激的牛血单核细胞中一氧化氮和肿瘤坏死因子-α 产生的不同影响。

Differential effects of heparin on NO and tumor necrosis factor-alpha production in bovine blood mononuclear cells stimulated with Salmonella typhimurium lipopolisaccharide.

作者信息

Chełmońska-Soyta A, Zimecki M, Nowacki W, Nikołajczuk M

机构信息

Department of Veterinary Prevention and Immunology, Faculty of Veterinary Medicine, Agriculture University of Wrocław, Poland.

出版信息

Comp Immunol Microbiol Infect Dis. 2001 Jul;24(3):151-64. doi: 10.1016/s0147-9571(00)00024-2.

DOI:10.1016/s0147-9571(00)00024-2

PMID:11440189

Abstract

We investigated the influence of heparin, one of the extracellular matrix (ECM) components, on nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) production by bovine peripheral blood mononuclear cells (PBMC) and monocytes left to adhere for 2 (freshly adherent monocytes) and 48 h (resting monocytes), activated with Salmonella typhimurium lipopolysaccharide (LPS). After 24-h stimulation with LPS, heparin (100 microg/ml) increased (by about 40%) NO production by peripheral blood mononuclear cells and by freshly adherent monocytes. However, it did not change NO synthesis by the resting monocytes. Unlike its influence on NO level, heparin diminished TNF-alpha production by PBMC and monocytes stimulated with LPS. Microscopical examination of PBMC stained with biotin-labeled heparin, showed that both lymphocytes and monocytes were able to bind this glycosaminoglycan. We suggest that heparin, as a component of ECM, modulates the early response of monocytes to exogenous stimuli.

摘要

我们研究了细胞外基质（ECM）成分之一的肝素，对经鼠伤寒沙门氏菌脂多糖（LPS）激活的牛外周血单个核细胞（PBMC）以及贴壁2小时（新鲜贴壁单核细胞）和48小时（静息单核细胞）的单核细胞产生一氧化氮（NO）和肿瘤坏死因子-α（TNF-α）的影响。在用LPS刺激24小时后，肝素（100微克/毫升）使外周血单个核细胞和新鲜贴壁单核细胞的NO生成增加（约40%）。然而，它并未改变静息单核细胞的NO合成。与它对NO水平的影响不同，肝素减少了经LPS刺激的PBMC和单核细胞的TNF-α生成。用生物素标记的肝素对PBMC进行显微镜检查显示，淋巴细胞和单核细胞都能够结合这种糖胺聚糖。我们认为，肝素作为ECM的一种成分，可调节单核细胞对外源刺激的早期反应。