急性苯丙胺处理或束缚应激后大鼠血浆催乳素反应及催乳素mRNA表达的品系差异。

Rat strain differences in responses of plasma prolactin and PRL mRNA expression after acute amphetamine treatment or restraint stress.

作者信息

Klenerova V, Sida P, Hynie S, Jurcovicova J

机构信息

Department of Pharmacology, First Medical Faculty, Charles University, Prague, Czech Republic.

出版信息

Cell Mol Neurobiol. 2001 Feb;21(1):91-100. doi: 10.1023/a:1007177430146.

DOI:10.1023/a:1007177430146

PMID:11440201

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11533817/

Abstract

The aim of this study was to compare the effects of acute amphetamine (AMPH) treatment and restraint stress on plasma level of prolactin (PRL) and PRL mRNA expression in the adenohypophysis in Sprague-Dawley and Lewis male rats, the latter known to have a deficient hypothalamo-pituitary-adrenal (HPA) axis. 2. Both restraint stress and AMPH treatment (i.p. in a dose of 8 mg/kg of b.w.) were applied 15 or 30 min before termination of the experiment. Plasma PRL and corticosterone (CORT) were determined by radioimmunoassay. PRL mRNA expression was estimated by a dot-blot hybridization. 3. Restraint stress and AMPH treatment induced a significant increase in the CORT plasma level, as an indicator of stress response. Compared to Sprague-Dawley rats, the magnitude of CORT increase after both stimuli was significantly lower in Lewis rats. 4. Although restraint stress significantly increased the PRL plasma levels in both rat strains, AMPH treatment reduced the PRL levels in both rat strains. However, the changes of PRL plasma levels had another pattern in Lewis rats than in Sprague-Dawley rats. Control plasma PRL levels were significantly higher in Lewis rats, and in this rat strain AMPH treatment for 30 min increased the PRL levels as compared to the values obtained after AMPH treatment for 15 min. 5. Expression of PRL mRNA in adenohypophysis by restraint stress and AMPH treatment had a similar pattern. After a 15-min lasting restraint stress, the expression of PRL mRNA was decreased insignificantly in both rat strains. AMPH treatment induced in Sprague-Dawley rats a significant decrease of PRL mRNA after a 15-min interval while after 30 min there was a significant increase. However, in Lewis rats AMPH failed to significantly change PRL mRNA. 6. The results from the present study indicate that the mechanisms mediating the effects of acute restraint stress and acute AMPH treatment differ in PRL response in Sprague-Dawley and Lewis male rat strains. Differences in the observed responses in Lewis rats could be related to the deficient activity of HPA axis in this rat strain.

摘要

本研究的目的是比较急性苯丙胺（AMPH）处理和束缚应激对Sprague-Dawley和Lewis雄性大鼠垂体前叶催乳素（PRL）血浆水平及PRL mRNA表达的影响，后者已知下丘脑-垂体-肾上腺（HPA）轴功能缺陷。2. 在实验结束前15或30分钟施加束缚应激和AMPH处理（腹腔注射，剂量为8 mg/kg体重）。通过放射免疫分析法测定血浆PRL和皮质酮（CORT）。通过斑点印迹杂交估计PRL mRNA表达。3. 束缚应激和AMPH处理导致CORT血浆水平显著升高，作为应激反应的指标。与Sprague-Dawley大鼠相比，两种刺激后Lewis大鼠CORT升高的幅度显著更低。4. 尽管束缚应激显著增加了两种大鼠品系的PRL血浆水平，但AMPH处理降低了两种大鼠品系的PRL水平。然而，Lewis大鼠PRL血浆水平的变化模式与Sprague-Dawley大鼠不同。Lewis大鼠的对照血浆PRL水平显著更高，并且在该大鼠品系中，与AMPH处理15分钟后获得的值相比，AMPH处理30分钟会增加PRL水平。5. 束缚应激和AMPH处理对垂体前叶PRL mRNA表达的模式相似。持续15分钟的束缚应激后，两种大鼠品系PRL mRNA的表达均无显著下降。AMPH处理在Sprague-Dawley大鼠中15分钟后导致PRL mRNA显著下降，而30分钟后则显著增加。然而，在Lewis大鼠中，AMPH未能显著改变PRL mRNA。6. 本研究结果表明，在Sprague-Dawley和Lewis雄性大鼠品系中，介导急性束缚应激和急性AMPH处理作用的机制在PRL反应方面存在差异。Lewis大鼠中观察到的反应差异可能与该大鼠品系中HPA轴的功能缺陷有关。