通过高效液相色谱法改进多胺及其共轭物的丹磺酰化衍生物分析
Improvement of the analysis of dansylated derivatives of polyamines and their conjugates by high-performance liquid chromatography.
作者信息
Fontaniella B, Mateos J L, Vicente C, Legaz M E
机构信息
Department of Plant Physiology, Faculty of Biology, Complutense University, Madrid, Spain.
出版信息
J Chromatogr A. 2001 Jun 15;919(2):283-8. doi: 10.1016/s0021-9673(01)00841-x.
The paper described a method for improving the hydrolysis of conjugated polyamines in PH fraction, isolated from the lichen Evernia prunastri, as well as the optimization of dansylation procedure of these polyamines on the basis of the pH value to which derivatization is achieved. Dansylated polyamines have been later separated by high-performance liquid chromatography (HPLC) using a gradient elution. Hydrolysis of conjugates requires acid treatment at room temperature rather than at 110 degrees C, as usually described. Dansylation is improved at high pH values, whereas removal of phenolics (mainly evernic acid), from the conjugates requires low pH values.
该论文描述了一种改进从地衣扁枝衣中分离出的PH级分中共轭多胺水解的方法，以及基于实现衍生化的pH值对这些多胺的丹磺酰化程序进行优化。随后，丹磺酰化的多胺通过高效液相色谱（HPLC）采用梯度洗脱进行分离。与通常描述的在110℃进行酸处理不同，共轭物的水解需要在室温下进行酸处理。丹磺酰化在高pH值下得到改善，而从共轭物中去除酚类物质（主要是扁枝衣酸）则需要低pH值。
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