Rogacheva S M, Ignatov O V
Saratov State University, Saratov, 410026 Russia.
Prikl Biokhim Mikrobiol. 2001 May-Jun;37(3):326-31.
The respiratory activity of Rhodococcus rhodochrous M8 cells containing nitrile hydratase and amidase was studied in the presence of nitriles and amides of carbonic acids. Culturing of cells with acrylonitrile and acrylamide yielding their maximum respiratory activity was studied. The optimum conditions for measurements and maintenance of respiratory activity were found. Curves for the linear concentration dependence of cell respiratory activity on 0.01-0.5 mM acrylonitrile, 0.025-1.0 mM acetonitrile, and 0.01-0.1 mM acrylamide were plotted. The selectivity of cell respiratory activity for some substrates was analyzed.
在碳酸腈和酰胺存在的情况下,研究了含有腈水合酶和酰胺酶的红平红球菌M8细胞的呼吸活性。研究了用丙烯腈和丙烯酰胺培养细胞以产生其最大呼吸活性的情况。发现了测量和维持呼吸活性的最佳条件。绘制了细胞呼吸活性对0.01 - 0.5 mM丙烯腈、0.025 - 1.0 mM乙腈和0.01 - 0.1 mM丙烯酰胺的线性浓度依赖性曲线。分析了细胞呼吸活性对某些底物的选择性。