Ma X F, Ross K, Gustafson J P
Plant Science Unit, University of Missouri, Columbia 65211, USA.
Genome. 2001 Jun;44(3):401-12.
Using wheat ditelosomic lines and in situ hybridization of biotin-labelled DNA probes, 18 restriction fragment length polymorphism (RFLP) markers were physically located on homoeologous groups 1 and 3 chromosomes of wheat. Most of the markers hybridized to chromosome arms in a physical order concordant with the genetic maps. A majority of the markers studied were clustered in non-C-banded, distal euchromatic areas, indicating the presence of recombination hot spots and cold spots in those regions. However, on IBS the markers were well dispersed, which could be due to the abundance of heterochromatin throughout the arm. An inversion between Xpsr653 and Xpsr953 was observed on 1AL. One new Xpsr688 locus, approximately 20-26% from the centromere, was found on 1AS and 1BS. The physical location of Xpsr170 on group 3 chromosomes probably represents an alternative to the loci on the genetic map. Finally, Xpsr313 was mapped to two physical loci on IDL. Five markers were located to bins consistent with the deletion-based physical maps.
利用小麦端体线和生物素标记的DNA探针进行原位杂交，将18个限制性片段长度多态性（RFLP）标记定位在小麦1和3部分同源群染色体上。大多数标记以与遗传图谱一致的物理顺序与染色体臂杂交。所研究的大多数标记聚集在非C带的远端常染色质区域，表明这些区域存在重组热点和冷点。然而，在IBS上标记分布良好，这可能是由于整个染色体臂上异染色质丰富。在1AL上观察到Xpsr653和Xpsr953之间的倒位。在1AS和1BS上发现了一个新的Xpsr688位点，距着丝粒约20 - 26%。Xpsr170在3部分同源群染色体上的物理定位可能是遗传图谱上这些位点的替代。最后，Xpsr313被定位到1DL上的两个物理位点。五个标记被定位到与基于缺失的物理图谱一致的 bins 中。
