SPARC在脂肪组织中的表达及其在冠心病患者血浆中的浓度升高。

The expression of SPARC in adipose tissue and its increased plasma concentration in patients with coronary artery disease.

作者信息

Takahashi M, Nagaretani H, Funahashi T, Nishizawa H, Maeda N, Kishida K, Kuriyama H, Shimomura I, Maeda K, Hotta K, Ouchi N, Kihara S, Nakamura T, Yamashita S, Matsuzawa Y

机构信息

Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.

出版信息

Obes Res. 2001 Jul;9(7):388-93. doi: 10.1038/oby.2001.50.

DOI:10.1038/oby.2001.50

PMID:11445660

Abstract

OBJECTIVE

Adipocytes secrete various cytokines and matrix proteins. Several of them precipitate in obesity-associated diseases, including atherosclerosis. In the current study, we have examined the expression of secreted protein, acidic and rich in cysteine (SPARC) in adipose tissue and its significance in obesity and coronary artery disease (CAD).

RESEARCH METHODS AND PROCEDURES

The SPARC mRNA expressions both in vivo and in vitro were detected by Northern blot analysis. Plasma SPARC concentrations were measured by enzyme immunosorbent assay. First, we investigated the plasma SPARC levels of 88 unrelated adult Japanese subjects (62 men and 26 women; average age: [+/- SD] 50 +/- 12 years; body mass index [BMI]: 16 to 46 kg/m(2)). Additionally 31 subjects with CAD diagnosed by coronary angiography (20 men and 11 women) were also investigated.

RESULTS

Human adipose tissues expressed abundant SPARC mRNA. SPARC expression in adipose tissues was upregulated in obese db/db mice. Markedly enhanced expression of SPARC mRNA was observed in 3T3-L1 fibroblasts during adipocyte differentiation. Consistent with these results, plasma SPARC levels proved a positive correlation with BMI in humans (r = 0.27; p < 0.01). Interestingly, plasma SPARC concentrations were significantly elevated in age- and BMI-matched subjects with CAD (p < 0.05).

DISCUSSION

SPARC was expressed in adipose tissues and its expression was enhanced in obese mice. In human, plasma SPARC levels were elevated in obesity and CAD patients. This elevated SPARC may be involved in the progression of CAD.

摘要

目的

脂肪细胞分泌多种细胞因子和基质蛋白。其中一些与肥胖相关疾病有关，包括动脉粥样硬化。在本研究中，我们检测了脂肪组织中富含半胱氨酸的酸性分泌蛋白（SPARC）的表达及其在肥胖和冠状动脉疾病（CAD）中的意义。

研究方法与步骤

通过Northern印迹分析检测体内和体外SPARC mRNA的表达。采用酶联免疫吸附测定法测量血浆SPARC浓度。首先，我们调查了88名无亲缘关系的成年日本受试者（62名男性和26名女性；平均年龄：[±标准差]50±12岁；体重指数[BMI]：16至46 kg/m²）的血浆SPARC水平。此外，还对31名经冠状动脉造影诊断为CAD的受试者（20名男性和11名女性）进行了调查。

结果

人体脂肪组织表达丰富的SPARC mRNA。肥胖的db/db小鼠脂肪组织中SPARC的表达上调。在3T3-L1成纤维细胞分化为脂肪细胞的过程中，观察到SPARC mRNA的表达明显增强。与这些结果一致，人类血浆SPARC水平与BMI呈正相关（r = 0.27；p < 0.01）。有趣的是，在年龄和BMI匹配的CAD受试者中，血浆SPARC浓度显著升高（p < 0.05）。