Castrop H, Kurtz A
Institut für Physiologie, Universität Regensburg, Germany.
J Hypertens. 2001 Jul;19(7):1223-31. doi: 10.1097/00004872-200107000-00007.
Several indications exist to suggest that an impaired production of nitric oxide might have a role in the development of salt-sensitive hypertension.
To examine whether the gene expression of the nitric oxide synthases (NOS) is altered in the salt-sensitive Dahl rat compared with that in the salt-resistant Dahl rat.
The abundance of NOS mRNA was measured by RNase protection assay in different organs of salt-resistant and salt-sensitive Dahl rats. In addition, the zonal expression of NOS genes in the kidney under salt load and salt restriction was determined.
The abundance of endothelial NOS mRNA was similar between the salt-resistant and salt-sensitive Dahl rat strains in all organs. Inducible NOS mRNA was not detectable by RNase protection assay in any organ. Neuronal NOS (nNOS) mRNA expression, however, was about 50% lower in brain and kidney of salt-sensitive Dahl rats than in salt-resistant Dahl rats. Within the kidney, nNOS mRNA levels were significantly decreased in salt-sensitive Dahl rats compared with those in salt-resistant Dahl rats, in cortex, outer and inner medulla (50, 40 and 30%, respectively) under all dietary conditions. A comparison of renal nNOS gene expression in Dahl rats with that in salt-insensitive Sprague- Dawley rats revealed that the abundance of renal nNOS was similar in salt-sensitive Dahl and Sprague-Dawley rats, but was increased in salt-resistant Dahl rats relative to that in Sprague-Dawley rats.
These data suggest that nNOS gene expression is increased in salt-resistant Dahl rats compared with that in salt-sensitive Dahl rats. This increased nNOS expression of the salt-resistant Dahl strain might play a part in compensating for a defect of renal salt excretion in the Dahl strains.
有多项迹象表明，一氧化氮生成受损可能在盐敏感性高血压的发生发展中起作用。
研究与盐抵抗性Dahl大鼠相比，盐敏感性Dahl大鼠中一氧化氮合酶（NOS）的基因表达是否发生改变。
采用核糖核酸酶保护分析法检测盐抵抗性和盐敏感性Dahl大鼠不同器官中NOS mRNA的丰度。此外，还测定了盐负荷和限盐条件下肾脏中NOS基因的区域表达。
在所有器官中，盐抵抗性和盐敏感性Dahl大鼠品系之间内皮型NOS mRNA的丰度相似。核糖核酸酶保护分析法在任何器官中均未检测到诱导型NOS mRNA。然而，盐敏感性Dahl大鼠脑和肾中的神经元型NOS（nNOS）mRNA表达比盐抵抗性Dahl大鼠低约50%。在肾脏内部，与盐抵抗性Dahl大鼠相比，盐敏感性Dahl大鼠在所有饮食条件下，皮质、外髓和内髓中的nNOS mRNA水平均显著降低（分别为50%、40%和30%）。比较Dahl大鼠与盐不敏感的Sprague-Dawley大鼠的肾脏nNOS基因表达发现，盐敏感性Dahl大鼠和Sprague-Dawley大鼠的肾脏nNOS丰度相似，但盐抵抗性Dahl大鼠相对于Sprague-Dawley大鼠有所增加。
这些数据表明，与盐敏感性Dahl大鼠相比，盐抵抗性Dahl大鼠中nNOS基因表达增加。盐抵抗性Dahl品系中这种增加的nNOS表达可能在补偿Dahl品系中肾盐排泄缺陷方面发挥作用。
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