Piela-Smith T H, Liu X
Research Service, Veterans Administration Connecticut Healthcare System, Newington, Connecticut 06111, USA.
Cell Immunol. 2001 May 1;209(2):89-96. doi: 10.1006/cimm.2001.1797.
Previous studies have shown that extracts of the aromatic herb feverfew (Tanacetum parthenium) and one of its bioactive components, parthenolide, have anti-inflammatory properties in vivo and in vitro. We examined both crude feverfew extracts and purified parthenolide for their ability to modulate adhesion molecule expression in human synovial fibroblasts. Pretreatment of synovial fibroblasts with either feverfew extracts or purified parthenolide could inhibit the expression of intercellular adhesion molecule-1 (ICAM-1) induced by the cytokines IL-1 (up to 95% suppression), TNF-alpha (up to 93% suppression), and, less strongly, interferon-gamma (up to 39% suppression). Inhibition of ICAM-1 was dose and time dependent; as little as a 30-min pretreatment with feverfew resulted in inhibition of ICAM-1. The decrease in ICAM-1 expression was accompanied by a decrease in T-cell adhesion to the treated fibroblasts. Other herbal extracts with reported anti-inflammatory effects were similarly tested and did not decrease ICAM-1 expression. The modulation of adhesion molecule expression may be an additional mechanism by which feverfew mediates anti-inflammatory effects.
先前的研究表明，芳香草本植物小白菊（Tanacetum parthenium）的提取物及其生物活性成分之一小白菊内酯在体内和体外均具有抗炎特性。我们研究了小白菊粗提物和纯化的小白菊内酯对人滑膜成纤维细胞中黏附分子表达的调节能力。用小白菊提取物或纯化的小白菊内酯预处理滑膜成纤维细胞，可抑制细胞因子IL-1（抑制高达95%）、TNF-α（抑制高达93%）以及抑制作用较弱的干扰素-γ（抑制高达39%）诱导的细胞间黏附分子-1（ICAM-1）的表达。ICAM-1的抑制呈剂量和时间依赖性；用小白菊预处理仅30分钟即可导致ICAM-1受到抑制。ICAM-1表达的降低伴随着T细胞对经处理的成纤维细胞黏附的减少。对其他具有抗炎作用报道的草药提取物进行了类似测试，它们并未降低ICAM-1的表达。黏附分子表达的调节可能是小白菊介导抗炎作用的另一种机制。
