Kindermann J, Kübber-Heiss A, Kerschbaumer P, Nowotny N
Institute of Virology, University of Veterinary Sciences, Vienna, Austria.
Arch Virol. 2001;146(5):1021-35. doi: 10.1007/s007050170133.
Two reptilian paramyxoviruses, isolated from a neotropical rattlesnake (neotropical virus, NTV, ATCC VR-1408) and a bush viper (bush viper virus, BVV, ATCC VR- 1409), respectively, were analysed to determine their taxonomic position among other reptilian paramyxoviruses investigated previously by Ahne et al.. A 679 bp long region of the hemagglutinin-neuraminidase (HN) gene and a 627 bp long region of the large (L) gene were reverse transcribed, amplified by polymerase chain reaction (PCR), and sequenced. The deduced amino acid sequences were compared to mammalian paramyxoviruses belonging to the genera Respirovirus and Rubulavirus. The deduced amino acid sequences revealed 58.9 to 62% homology for the partial L protein and 41% to 47.1% homology for the partial HN protein. For phylogenetic analyses, a 518 bp L gene and a 352 bp HN gene fragment were used, both generating similar trees consisting of two distinct main groups, and some intermediate isolates. BVV clustered within group "b" while NTV clustered together with the intermediate ophidian paramyxovirus isolate Crot2-OH90.
分别从一种新热带响尾蛇（新热带病毒，NTV，ATCC VR - 1408）和一种树蝰（树蝰病毒，BVV，ATCC VR - 1409）中分离出两种爬行动物副粘病毒，对其进行分析以确定它们在Ahne等人之前研究的其他爬行动物副粘病毒中的分类地位。对血凝素 - 神经氨酸酶（HN）基因的一个679 bp长的区域和大（L）基因的一个627 bp长的区域进行逆转录，通过聚合酶链反应（PCR）扩增并测序。将推导的氨基酸序列与属于呼吸病毒属和腮腺炎病毒属的哺乳动物副粘病毒进行比较。推导的氨基酸序列显示部分L蛋白的同源性为58.9%至62%，部分HN蛋白的同源性为41%至47.1%。为了进行系统发育分析，使用了一个518 bp的L基因和一个352 bp的HN基因片段，两者都生成了由两个不同的主要组和一些中间分离株组成的相似树状图。BVV聚集在“b”组内，而NTV与中间的蛇类副粘病毒分离株Crot2 - OH90聚集在一起。
