抗猪圆环病毒2单克隆抗体的制备、特性鉴定及应用
Production, characterisation and applications of monoclonal antibodies to porcine circovirus 2.
作者信息
McNeilly F, McNair I, Mackie D P, Meehan B M, Kennedy S, Moffett D, Ellis J, Krakowka S, Allan G M
机构信息
Virology Department, Department of Agriculture and Rural Development for N. Ireland, Belfast, UK.
出版信息
Arch Virol. 2001;146(5):909-22. doi: 10.1007/s007050170124.
The production, preliminary characterisation and applications of monoclonal antibodies (mabs) against six porcine circovirus 2 isolates are described. A total of 14 stable hybridomas were produced, of which 7 were characterised. All of the mabs characterised were of IgG isotype. All the mabs tested reacted by IIF with acetone-fixed cell cultures infected with PCV2 isolates from Canada, France, Spain, Denmark, USA and UK. No cross-reactivity with a porcine circovirus 1 field isolate was demonstrated using the panel of mabs tested. In addition, one of the seven mabs tested demonstrated neutralising activity against PCV2 isolates from Canada and France. The use of selected PCV2-specific mabs for the development of virus detection methodologies is described.
本文描述了针对六种猪圆环病毒2型分离株的单克隆抗体（mabs）的制备、初步鉴定及应用。共产生了14个稳定的杂交瘤，其中7个进行了鉴定。所有鉴定的单克隆抗体均为IgG同种型。所有测试的单克隆抗体通过间接免疫荧光法（IIF）与感染了来自加拿大、法国、西班牙、丹麦、美国和英国的猪圆环病毒2型分离株的丙酮固定细胞培养物发生反应。使用所测试的单克隆抗体组未显示与猪圆环病毒1型田间分离株有交叉反应。此外，所测试的7个单克隆抗体中的一个对来自加拿大和法国的猪圆环病毒2型分离株表现出中和活性。本文还描述了使用选定的猪圆环病毒2型特异性单克隆抗体开发病毒检测方法的情况。
Zotero 插件