Allan G M, Mackie D P, McNair J, Adair B M, McNulty M S
Veterinary Sciences Division, Department of Agriculture, Stormont, Belfast, UK.
Vet Immunol Immunopathol. 1994 Nov;43(4):357-71. doi: 10.1016/0165-2427(94)90157-0.
The preparation of monoclonal antibodies (mAbs) to porcine circovirus is described. Preliminary characterisation was carried out on nine mAbs obtained from two fusions and included isotyping, virus neutralisation assays and indirect immunofluorescence staining patterns obtained following immunostaining of both a porcine circovirus (PCV)-persistently infected pig kidney (PK/15/W) and Vero (Vero-PCV) cell line. Significant differences in the staining patterns were observed in both cell lines which appeared to be dependent on the subculture status of the Vero-PCV cultures. The development of a mAb-based antigen capture enzyme linked immunosorbent assay (ELISA) as an aid to virus purification is also described. The use of mAbs for the detection of PCV antigen in cryostat sections from a pig experimentally infected with the virus leading to identification of the sites of replication of PCV is also reported.
本文描述了抗猪圆环病毒单克隆抗体（mAb）的制备。对从两次融合中获得的9种单克隆抗体进行了初步表征，包括亚型鉴定、病毒中和试验以及对猪圆环病毒（PCV）持续感染的猪肾（PK/15/W）细胞系和Vero（Vero-PCV）细胞系进行免疫染色后获得的间接免疫荧光染色模式。在两种细胞系中均观察到染色模式存在显著差异，这似乎取决于Vero-PCV培养物的传代状态。本文还描述了基于单克隆抗体的抗原捕获酶联免疫吸附测定（ELISA）在病毒纯化中的应用。还报道了使用单克隆抗体检测实验感染该病毒的猪的冷冻切片中的PCV抗原，从而确定PCV的复制位点。
