Weimer J, Koehler M R, Wiedemann U, Attermeyer P, Jacobsen A, Karow D, Kiechl M, Jonat W, Arnold N
Oncology Laboratory, Gynecology and Obstetrics Clinic, Christian-Albrechts University Kiel, Germany.
Chromosome Res. 2001;9(5):395-402. doi: 10.1023/a:1016735618513.
A technique disclosing most information about chromosome modifications is the technique of choice for the analysis of chromosome alterations. The newly developed method for microdissection of fluorescence-labeled chromosomes (FISH-MD) can improve upon this expectation in combination with 24-color spectral karyotyping (SKY). The highly efficient way to detect chromosome modifications by SKY and the detailed specification of aberrant chromosomes by FISH-MD prompted us to use both techniques in a combined approach called SKY-MD. First, an overview of chromosomal aberrations is obtained by spectral karyotyping and subsequently the derivative chromosomes recognized are characterized in a highly specific manner by microdissection and reverse painting. A small quantity of isolated material dissected directly from a 24-color metaphase is sufficient to obtain very detailed information about the chromosome regions and the breakpoints involved in the derivative chromosomes. Therefore, the combination of spectral karyotyping and microdissection in one procedure, and reverse painting can characterize chromosomal aberrations with a degree of specificity hitherto unknown from individual karyotyping experiments. In this article we compare the efficiency of both the SKY technique and that of classical microdissection with the efficiency obtained by SKY-MD.
一种能揭示最多染色体修饰信息的技术是用于分析染色体改变的首选技术。新开发的荧光标记染色体显微切割技术（FISH-MD）与24色光谱核型分析（SKY）相结合，有望超越这一预期。SKY检测染色体修饰的高效方法以及FISH-MD对异常染色体的详细鉴定，促使我们将这两种技术结合使用，形成一种名为SKY-MD的联合方法。首先，通过光谱核型分析获得染色体畸变的概述，随后通过显微切割和反向涂色以高度特异性的方式对识别出的衍生染色体进行表征。直接从24色中期相中分离出的少量材料，就足以获取有关衍生染色体所涉及的染色体区域和断点的非常详细的信息。因此，在一个程序中将光谱核型分析和显微切割以及反向涂色相结合，能够以单个核型分析实验迄今未知的特异性程度来表征染色体畸变。在本文中，我们将SKY技术、经典显微切割技术的效率与SKY-MD所获得的效率进行了比较。
