Weimer J, Kiechle M, Arnold N
Oncology Laboratory, Gynecology and Obstetrics Clinic, Christian-Albrechts University Kiel, Germany.
Cytogenet Cell Genet. 2000;88(1-2):114-8. doi: 10.1159/000015502.
We combined the techniques of fluorescence in situ hybridization (FISH) and chromosomal microdissection in one experiment (FISH-MD). This novel method permits rapid identification of the composition, origin, and breakpoints of rearranged chromosomes. Rearranged chromosomes are first identified by multicolor-FISH, then the fluorophore-labeled derivative chromosomes are directly isolated by microdissection and reverse painted to identify the breakpoints.
我们在一个实验中结合了荧光原位杂交（FISH）技术和染色体显微切割技术（FISH-MD）。这种新方法能够快速鉴定重排染色体的组成、起源和断点。首先通过多色FISH鉴定重排染色体，然后通过显微切割直接分离荧光团标记的衍生染色体，并进行反向涂染以鉴定断点。
