Pessina F, Kalfin R, Sgaragli G
Istituto di Scienze Farmacologiche, Via E.S. Piccolomini 170, 53100 Siena, Italy.
Eur J Pharmacol. 2001 Jul 6;423(2-3):229-33. doi: 10.1016/s0014-2999(01)01108-6.
Vasoactive intestinal peptide (VIP) was tested for its capability to protect the intrinsic nerves of guinea-pig urinary bladder from damage due to anoxia/glucopenia and reperfusion. Guinea-pig detrusor strips were mounted for tension recording in small organ baths and the nerves were subjected to electric field stimulation. VIP (0.3 microM) improved significantly the response of strips to electrical field stimulation either during anoxia/glucopenia or thereafter during reperfusion, as compared to untreated tissues. The antioxidant activity of VIP assessed as its capability to scavenge peroxyl radicals during linoleic acid oxidation corresponded to 6.42+/-0.13 pIC(50) M, i.e. close to the concentration proved to protect strips against the anoxic--glucopenic and reperfusion damage.
对血管活性肠肽（VIP）保护豚鼠膀胱内在神经免受缺氧/低血糖及再灌注损伤的能力进行了测试。将豚鼠逼尿肌条安装在小型器官浴槽中进行张力记录，并对神经施加电场刺激。与未处理的组织相比，VIP（0.3微摩尔）在缺氧/低血糖期间或之后的再灌注期间均显著改善了肌条对电场刺激的反应。VIP的抗氧化活性通过其在亚油酸氧化过程中清除过氧自由基的能力来评估，相当于6.42±0.13 pIC50 M，即接近被证明可保护肌条免受缺氧-低血糖及再灌注损伤的浓度。
