普通变形杆菌O39酸性O-特异性多糖的结构，其含有5,7-二乙酰氨基-3,5,7,9-四脱氧-L-甘油-L-甘露糖-壬-2-酮糖酸。

Structure of the acidic O-specific polysaccharide from Proteus vulgaris O39 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid.

作者信息

Kondakova A N, Perepelov A V, Bartodziejska B, Shashkov A S, Senchenkova S N, Wykrota M, Knirel Y A, Rozalski A

机构信息

N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospeckt 47, Moscow 119991, Russia.

出版信息

Carbohydr Res. 2001 Jul 12;333(3):241-9. doi: 10.1016/s0008-6215(01)00145-8.

DOI:10.1016/s0008-6215(01)00145-8

PMID:11448686

Abstract

The O-specific polysaccharide of Proteus vulgaris O39 was found to contain a new acidic component of Proteus lipopolysaccharides, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid (di-N-acetylpseudaminic acid, Pse5Ac7Ac). The following structure of the polysaccharide was determined by NMR spectroscopy, including 2D 1H,(1)H COSY, TOCSY, ROESY, and 1H,(13)C HMQC experiments, along with selective cleavage of the polysaccharide by solvolysis with anhydrous trifluoromethanesulfonic (triflic) acid: -->8)-beta-Psep5Ac7Ac-(2-->3)-alpha-L-FucpNAc-(1-->3)-alpha-D-GlcpNAc-(1--> The structure established is unique among the O-specific polysaccharides, which is in accordance with classification of the strain studied into a separate Proteus serogroup.

摘要

普通变形杆菌O39的O-特异性多糖被发现含有变形杆菌脂多糖的一种新的酸性成分，即5,7-二乙酰氨基-3,5,7,9-四脱氧-L-甘油-L-甘露-壬-2-酮糖酸（二-N-乙酰假氨基糖酸，Pse5Ac7Ac）。通过核磁共振光谱法，包括二维1H,(1)H COSY、TOCSY、ROESY和1H,(13)C HMQC实验，以及用无水三氟甲磺酸（三氟磺酸）进行溶剂解对多糖进行选择性裂解，确定了多糖的以下结构：→8)-β-Psep5Ac7Ac-(2→3)-α-L-FucpNAc-(1→3)-α-D-GlcpNAc-(1→ 所确定的结构在O-特异性多糖中是独特的，这与所研究菌株被分类到一个单独的变形杆菌血清群相一致。