Fujita N, Hasegawa H, Taira T
Laboratory of Plant Genes and Physiology, College of Agriculture, Osaka Prefecture University, Sakai, 599-8531, Osaka, Japan
Plant Sci. 2001 Mar;160(4):595-602. doi: 10.1016/s0168-9452(00)00408-8.
A waxy mutant of diploid wheat (Triticum monococcum L.) was isolated by screening M(3) seeds derived from 1% ethyl methanesulfonate (EMS) mutagenized materials with KI-I(2) staining of endosperm starch. This mutant was controlled by a single waxy gene that completely lacked amylose, the 59-kDa waxy protein and the granule-bound starch synthase I (GBSS I) activity in the endosperm. Moreover, the T. monococcum exhibited the gene dosage effects in terms of amylose content, the amount of waxy protein and the GBSS activity. The chain length distribution of amylopectin of the waxy mutant endosperm starch slightly differed from that of the wild type; the long chains of DP>==19 were more enriched in the waxy amylopectin than those in the wild type. The mutant can be an important material for studies on starch metabolism and breeding in Triticum species.
通过对1%甲磺酸乙酯（EMS）诱变处理材料的M(3)种子进行胚乳淀粉碘-碘化钾染色筛选，分离得到了二倍体小麦（一粒小麦Triticum monococcum L.）的一个蜡质突变体。该突变体由单个蜡质基因控制，其胚乳中完全缺乏直链淀粉、59 kDa蜡质蛋白和颗粒结合淀粉合酶I（GBSS I）活性。此外，一粒小麦在直链淀粉含量、蜡质蛋白量和GBSS活性方面表现出基因剂量效应。蜡质突变体胚乳淀粉的支链淀粉链长分布与野生型略有不同；蜡质支链淀粉中DP>==19的长链比野生型更丰富。该突变体可作为小麦淀粉代谢和育种研究的重要材料。
