Nechipurenko Iu D, Strel'tsov S A, Evdokimov Iu M
Engelgardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 117984 Russia.
Biofizika. 2001 May-Jun;46(3):428-35.
A thermodynamic model of the formation of bridges consisting of alternating daunomycin molecules and copper ions and connecting neighboring nucleic acid molecules in a particle of a liquid crystalline dispersion was constructed. The model is based on the conception that ligands are adsorbed on lattices of reaction centers which are formed in a liquid crystal at a particular spatial arrangement of adjacent nucleic acid molecules (phasing). Equations were derived that describe typical experimentally obtained S-shaped dependences of bridge concentration on the concentration of copper ions and daunomycin molecules in an initial solution. It was shown that dependences of this kind take place in two variants of the adsorption model: when the binding of daunomycin with adjacent nucleic acid molecules is considered to be independent on the formation of bridges and when bridges compete with single daunomycin molecules for the sites on adjacent nucleic acid molecules.
构建了一个热力学模型，用于描述在液晶分散体颗粒中，由交替排列的柔红霉素分子和铜离子组成的桥的形成过程，这些桥连接相邻的核酸分子。该模型基于这样的概念：配体吸附在反应中心的晶格上，这些反应中心是在液晶中由相邻核酸分子的特定空间排列（相位）形成的。推导了一些方程，这些方程描述了实验中典型得到的桥浓度对初始溶液中铜离子和柔红霉素分子浓度的S形依赖性。结果表明，这种依赖性在吸附模型的两种变体中都会出现：一种是认为柔红霉素与相邻核酸分子的结合独立于桥的形成；另一种是桥与单个柔红霉素分子竞争相邻核酸分子上的位点。
