[在西伯利亚分离出的霍乱弧菌0139流行病学重要菌株标志物的聚合酶链反应检测]
[PCR-detection of markers for epidemiologically-significant strains of Vibrio cholerae 0139, isolated in Siberia].
作者信息
Balakhonov S V, Mironova L V, Maramovich A S, Ganin V S, Urbanovich L Ia, Pogorelov V I
机构信息
Plague Control Institute of Siberia and Far East, Irkutsk.
出版信息
Mol Gen Mikrobiol Virusol. 2001(2):24-6.
Fourteen V. cholerae 0139 strains were isolated in 1996-1999 in Siberia from the Ob river (Novosibirsk) and bogs and lakes (Irkutsk). The strains were tested in PCR for the key virulence determinants (ctx AB, tcp, acf). The genomes lacked these elements, and therefore the strains were acknowledged avirulent. The results correlate completely with the data of phenotypical analysis, characterizing the pathogenic characteristics of isolated strains.
1996年至1999年期间，在西伯利亚的鄂毕河（新西伯利亚）以及沼泽和湖泊（伊尔库茨克）分离出了14株霍乱弧菌O139菌株。对这些菌株进行了关键毒力决定因素（ctx AB、tcp、acf）的PCR检测。这些菌株的基因组缺乏这些元件，因此被认为是无毒的。结果与表型分析数据完全相关，表型分析表征了分离菌株的致病特征。
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