Topical application of brain derived neurotrophic factor influences upregulation of constitutive isoform of heme oxygenase in the spinal cord following trauma an experimental study using immunohistochemistry in the rat.

Influence of brain derived neurotrophic factor (BDNF) on carbon monoxide (CO) production following spinal cord injury was examined using expression of the constitutive isoform of the enzyme hemeoxygenase-2 (HO-2) in a rat model. A longitudinal incision of the right dorsal horn on the T10-11 segment markedly increased the HO-2 immunostaining in the cord at 5 h. At this time period, breakdown of the blood-spinal cord barrier (BSCB) and edema formation were quite prominent. Repeated topical application of BDNF (20 microliters of a 1 microgram/ml solution) over the exposed surface of the cord significantly attenuated the edematous expansion of the cord and the disturbances in the BSCB permeability. In BDNF-treated rats, expression of HO-2 immunoreactivity was considerably reduced. These results strongly suggest that BDNF is neuroprotective in spinal trauma and this growth factor has the capacity to attenuate CO production by downregulating HO-2 expression.