细胞壁肽聚糖分支酶MurM的改变会增加肺炎链球菌对高水平青霉素和头孢菌素的耐药性。
Alterations in MurM, a cell wall muropeptide branching enzyme, increase high-level penicillin and cephalosporin resistance in Streptococcus pneumoniae.
作者信息
Smith A M, Klugman K P
机构信息
Pneumococcal Diseases Research Unit, Department of Clinical Microbiology and Infectious Diseases, South African Institute for Medical Research, Johannesburg, South Africa.
出版信息
Antimicrob Agents Chemother. 2001 Aug;45(8):2393-6. doi: 10.1128/AAC.45.8.2393-2396.2001.
Abstract
We report that alteration in MurM, an enzyme involved in the biosynthesis of branched-stem cell wall muropeptides, is required for maximal expression of penicillin and cefotaxime resistance in the pneumococcus. Hungarian isolate 3191 (penicillin MIC, 16 microg/ml; cefotaxime MIC, 4 microg/ml) was a source of donor DNA in transformation experiments. Penicillin-binding protein DNA was insufficient to transform recipient strain R6 to full resistance. Further transformation with altered murM DNA was required for full expression of donor penicillin and cefotaxime resistance.
摘要
我们报告称，肺炎球菌中青霉素和头孢噻肟耐药性的最大表达需要参与支链干细胞壁肽聚糖生物合成的MurM酶发生改变。匈牙利分离株3191（青霉素MIC，16微克/毫升；头孢噻肟MIC，4微克/毫升）是转化实验中供体DNA的来源。青霉素结合蛋白DNA不足以将受体菌株R6转化为完全耐药。需要用改变的murM DNA进一步转化才能使供体青霉素和头孢噻肟耐药性完全表达。
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