从胎儿血清和肝癌血清中分离并鉴定人胎儿α-球蛋白（α1F）

Isolation and characterization of human foetal alpha-globulin (alpha 1F) from foetal and hepatoma sera.

作者信息

Adinolfi A, Adinolfi M, Cohen S

机构信息

Paediatric Research Unit, Guy's Hospital Medical School, London, S.E. 1., Great Britain.

出版信息

Biochim Biophys Acta. 1971 Nov 19;251(2):197-207. doi: 10.1016/0005-2795(71)90103-6.

DOI:10.1016/0005-2795(71)90103-6

PMID:11452385

Abstract

The alpha 1F present in human foetal sera and the serum from a patient with hepatocellular carcinoma was isolated by immune precipitation using rabbit anti-alpha 1F. After labelling with 125I, some physicochemical properties of alpha 1F were investigated. The molecular weights of 125I-labelled alpha 1F isolated from foetal and hepatoma sera were 61,000 and 63,000, respectively. The protein was not dissociated in 6 M guanidine after complete reduction, showing that it contains a single peptide chain.

摘要

使用兔抗α1F通过免疫沉淀法分离人胎儿血清和一名肝细胞癌患者血清中的α1F。用125I标记后，研究了α1F的一些物理化学性质。从胎儿血清和肝癌血清中分离出的125I标记的α1F的分子量分别为61,000和63,000。完全还原后，该蛋白质在6M胍中未解离，表明它含有一条单一肽链。