French M J, Angus J C, Walton A G
Materials Science Center, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Biochim Biophys Acta. 1971 Dec 28;251(3):320-30. doi: 10.1016/0005-2795(71)90118-8.
A study of the conformation of collagen and gelatin in aqueous solution by Optical Self Beat Spectroscopy is reported. The translational diffusion coefficient of monomeric tropocollagen was experimentally measured from the half-width of the Rayleigh scattered radiation and the value obtained is shown to be in good agreement with that calculated from hydrodynamical theory for the tropocollagen rod. The Self Beat Spectrometer was also used to investigate the factors affecting the aggregation and flexing of molecules in dilute gelatin solutions and the gel-sol transition in more concentrated gelatin solutions.
报道了一项通过光学自差光谱法对胶原蛋白和明胶在水溶液中的构象进行的研究。从瑞利散射辐射的半高宽实验测量了单体原胶原蛋白的平移扩散系数，所得值与从原胶原棒的流体动力学理论计算得到的值显示出良好的一致性。自差光谱仪还用于研究影响稀明胶溶液中分子聚集和弯曲以及更浓明胶溶液中凝胶 - 溶胶转变的因素。
