Hering S, Isken E, Knabbe C, Janott J, Jost C, Pommer A, Muhr G, Schatz H, Pfeiffer A F
Department of Internal Medicine, BG-Kliniken Bergmannsheil, Ruhr-University, Bochum, Germany.
Exp Clin Endocrinol Diabetes. 2001;109(4):217-26. doi: 10.1055/s-2001-15109.
Transforming growth factor beta is one of the most abundant growth factors stored in bone. It is known as a potent regulator of osteoblast proliferation and differentiation as well as of production extracellular matrix. We established a highly specific RT-PCR in combination with HPLC for detection and quantification of TGFbeta1 and TGFbeta2 mRNA expression in 89 human bone samples. Levels of TGFbeta1 protein ranged between 27 and 580 ng/g bone (mean 188 +/- 15 ng/g; n=75) and for TGFbeta2 between 7.2 and 35 ng/g bone (mean 14.3 +/- 2.1 ng/g; n=57). TGFbeta1 and TGFbeta2 protein concentrations and TGFbeta isoform mRNA expression in bone were not significantly different between the sexes. TGFbeta isoform mRNA expression as well as protein content in bone declined age dependently. TGFbeta1 and TGFbeta2 protein and mRNA expression were different in bone samples from different sites of the skeleton indicating in part the regulation by mechanical stimuli. In contrast to TGFbeta1, TGFbeta2 mRNA expression was significantly enhanced in osteoarthritic bone compared to unaffected bone. These data are in concordance to previous results concerning the expression of TGFbeta3 in bone. In conclusion, the data suggest distinct patterns' of expression of the TGFbeta isoforms under physiological and pathological conditions in bone.
转化生长因子β是储存在骨骼中最丰富的生长因子之一。它是成骨细胞增殖、分化以及细胞外基质产生的有效调节因子。我们建立了一种高度特异性的逆转录聚合酶链反应（RT-PCR）并结合高效液相色谱法（HPLC），用于检测和定量89份人类骨样本中TGFβ1和TGFβ2 mRNA的表达。TGFβ1蛋白水平在27至580 ng/g骨之间（平均188±15 ng/g；n = 75），TGFβ2在7.2至35 ng/g骨之间（平均14.3±2.1 ng/g；n = 57）。骨骼中TGFβ1和TGFβ2蛋白浓度以及TGFβ亚型mRNA表达在性别之间无显著差异。骨骼中TGFβ亚型mRNA表达以及蛋白含量随年龄增长而下降。来自骨骼不同部位的骨样本中TGFβ1和TGFβ2蛋白及mRNA表达不同，这部分表明了机械刺激的调节作用。与TGFβ1相反，与未受影响的骨相比，骨关节炎骨中TGFβ2 mRNA表达显著增强。这些数据与先前关于骨骼中TGFβ3表达的结果一致。总之，数据表明在骨骼的生理和病理条件下，TGFβ亚型存在不同的表达模式。
