Patterson L J, Aberdeen A, Kone J, Haben M, Raymond M, Berkower I
Laboratory of Immunoregulation, Office of Vaccine Research and Review, FDA/National Institutes of Health, NIH Campus Bldg. 29, Bethesda, MD 20892, USA.
Biochem Biophys Res Commun. 2001 Jul 20;285(3):639-43. doi: 10.1006/bbrc.2001.5227.
We have identified an acceptor site on HIV gp120, where foreign protein sequences can be inserted while retaining the native conformation of gp120. The resulting hybrids showed dual antigenicity, normal glycosylation, and high affinity binding of the CD4 receptor. This site allows insertion of highly immunogenic proteins such as core antigen of hepatitis B virus. By combining the immunogenicity of the carrier protein with the antigenicity of gp120, these hybrids may lead to modified HIV-1 antigens with enhanced immunogenicity.
我们已在HIV gp120上确定了一个受体位点，可在此处插入外源蛋白序列，同时保持gp120的天然构象。所得的杂交体表现出双重抗原性、正常糖基化以及与CD4受体的高亲和力结合。该位点允许插入高度免疫原性的蛋白质，如乙型肝炎病毒核心抗原。通过将载体蛋白的免疫原性与gp120的抗原性相结合，这些杂交体可能会产生免疫原性增强的修饰HIV-1抗原。
