钙调蛋白在正常生长条件和有丝分裂饥饿状态下可保护细胞免于死亡，但在B细胞受体刺激时的细胞死亡过程中起介导作用。
Calmodulin protects cells from death under normal growth conditions and mitogenic starvation but plays a mediating role in cell death upon B-cell receptor stimulation.
作者信息
Schmalzigaug R, Ye Q, Berchtold M W
机构信息
Department of Molecular Cell Biology, Institute of Molecular Biology, University of Copenhagen, Denmark.
出版信息
Immunology. 2001 Jul;103(3):332-42. doi: 10.1046/j.1365-2567.2001.01259.x.
Calmodulin (CaM) is the main intracellular Ca2+ sensor protein responsible for mediating Ca2+ triggered processes. Chicken DT40 lymphoma B cells express CaM from the two genes, CaMI and CaMII. Here we report the phenotypes of DT40 cells with the CaMII gene knocked out. The disruption of the CaMII gene causes the intracellular CaM level to decrease by 60%. CaMII-/- cells grow more slowly and die more frequently as compared to wild type (wt) cells but do not exhibit significant differences in their cell cycle profile. Both phenotypes are more pronounced at reduced serum concentrations. Upon stimulation of the B-cell receptor (BCR), the resting Ca2+ levels remain elevated after the initial transient in CaMII-/- cells. Despite higher Ca2+ resting levels, the CaMII-/- cells are partially protected from BCR induced apoptosis indicating that CaM plays a dual role in apoptotic processes.
钙调蛋白（CaM）是主要的细胞内钙离子传感蛋白，负责介导钙离子触发的过程。鸡DT40淋巴瘤B细胞从CaMI和CaMII这两个基因表达钙调蛋白。在此我们报告了CaMII基因敲除的DT40细胞的表型。CaMII基因的破坏导致细胞内钙调蛋白水平降低60%。与野生型（wt）细胞相比，CaMII-/-细胞生长更缓慢，死亡更频繁，但它们的细胞周期分布没有显著差异。这两种表型在血清浓度降低时更为明显。在刺激B细胞受体（BCR）后，CaMII-/-细胞在最初的短暂变化后静息钙离子水平仍保持升高。尽管静息钙离子水平较高，但CaMII-/-细胞在一定程度上受到保护，免受BCR诱导的凋亡，这表明钙调蛋白在凋亡过程中起双重作用。
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