Kamata M, Hu J, Shibahara H, Nakagawa H
Research and Development Center, BML Incorporation, 1361-1 Matoba, Kawagoe-shi, Saitama 350-1101, Japan.
Int J Androl. 2001 Aug;24(4):225-31. doi: 10.1046/j.1365-2605.2001.00288.x.
The testicular isozyme of angiotensin-converting enzyme (ACE) is associated with male fertility. Spermatozoa from mice lacking ACE showed defects in transport within the oviducts and in binding to zonae pellucidae although the animals had normal sperm count, morphology and motility. In fact, unexplained infertility is difficult to be predicted by conventional parameters such as sperm count. We measured membrane testicular ACE activity in a sperm suspension in PBS and total testis ACE activity in spermatozoa by solubilization with Triton X-100. Total testis ACE activity and membrane testis ACE activity of the same subject were compared in 12 control subjects. We demonstrated that testicular ACE is stable in spermatozoa and the assay of testicular ACE activity is possible. Total testicular ACE activity was approximately twice the membrane testicular ACE activity in all of the subjects tested. The assay of testicular ACE activity in human spermatozoa could be a new method for the assessment of sperm function.
血管紧张素转换酶（ACE）的睾丸同工酶与男性生育能力有关。缺乏ACE的小鼠的精子在输卵管内的运输以及与透明带的结合方面存在缺陷，尽管这些动物的精子数量、形态和活力正常。事实上，传统参数如精子数量难以预测不明原因的不孕症。我们用PBS在精子悬液中测量了睾丸膜ACE活性，并通过用Triton X-100溶解精子来测量精子中的总睾丸ACE活性。在12名对照受试者中比较了同一受试者的总睾丸ACE活性和膜睾丸ACE活性。我们证明了睾丸ACE在精子中是稳定的，并且可以检测睾丸ACE活性。在所有测试的受试者中，总睾丸ACE活性约为膜睾丸ACE活性的两倍。检测人类精子中的睾丸ACE活性可能是一种评估精子功能的新方法。
